江苏农业学报2025，Vol.41Issue(4)：702-714,13.DOI:10.3969/j.issn.1000-4440.2025.04.009
健康广藿香与患病广藿香根际土壤微生物群落的结构
Microbial community structure in rhizosphere soil of healthy and diseased patchouli(Pogostemon cablin)
摘要
Abstract
To explore the structure and diversity of the rhizosphere soil microbial community of healthy and diseased Pog-ostemon cablin(patchouli),high-throughput sequencing technology was employed to sequence and conduct functional prediction analyses on the microbial communities in the rhizosphere soil of both healthy and diseased patchouli plants.The results indicated that after patchouli plants were diseased,the to-tal phosphorus content in the rhizosphere soil increased significantly(P＜0.05).A total of 801 fungal operational taxonomic units(OTUs)and 3 477 bacterial OTUs were i-dentified in the rhizosphere soil of both healthy and dis-eased patchouli plants.The number of fungi in the rhizo-sphere soil of diseased patchouli plants increased significantly,while the number of bacteria decreased.The composition of microbial phyla and genera in the rhizosphere soil of healthy and diseased patchouli plants remained unchanged,but differ-ences were observed in the relative abundance of some phyla and genera.After the plants were diseased,at the phylum lev-el,the relative abundances of Acidobacteria and Verrucomicrobia bacteria,as well as Ascomycota fungi decreased signifi-cantly,while the relative abundances of Firmicutes bacteria and Basidiomycota fungi increased significantly.At the genus level,the relative abundances of Paenarthrobacter,Ralstonia,and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium bacteria,as well as Talaromyces fungi increased significantly,while the relative abundances of Podospora,Conlarium and Arthrobotrys fungi decreased significantly.Correlation analysis results showed that there was an obvious difference in the cor-relation between the rhizosphere microbial phyla of healthy and diseased patchouli plants and soil environmental factors.In diseased patchouli plants,soil physicochemical indicators were correlated with the relative abundance of more bacterial phy-la,while in healthy plants,soil physicochemical indicators were correlated with the relative abundance of more fungal phy-la.FAPROTAX functional prediction results indicated that there were obvious differences in the relative abundance of four types of bacterial functions,including plant pathogen,ureolysis,aromatic hydrocarbon degradation and hydrocarbon degra-dation,between the rhizosphere soil of healthy and diseased patchouli plants.FUNGuild functional prediction results showed that there were significant differences in the relative abundances of pathological-saprophytic-symbiotic and symbiotic nutri-tional types of fungal nutritional types in the rhizosphere soil of healthy and diseased patchouli plants.The relative abun-dance of plant pathogens in diseased plants increased significantly.The functional prediction results suggested that there was a certain correlation between the occurrence of patchouli disease and changes in the relative abundance of pathogenic micro-organisms.The findings of this study can provide a theoretical basis for the prevention and control of patchouli diseases.关键词
广藿香/根际土壤/微生物群落/高通量测序/病害防治Key words
Pogostemon cablin/rhizosphere soil/microbial community/high-throughput sequencing/disease prevention and control分类
农业科技引用本文复制引用
许乐乐,黄倩敏,李永芹,王锂韫..健康广藿香与患病广藿香根际土壤微生物群落的结构[J].江苏农业学报,2025,41(4):702-714,13.基金项目
湛江市科技计划项目(2022A01054) （2022A01054）
广东省教育厅项目(2021KCXTD039) （2021KCXTD039）
