江苏农业学报2025，Vol.41Issue(4)：724-732,9.DOI:10.3969/j.issn.1000-4440.2025.04.011
急性低氧胁迫下中华绒螯蟹表皮基因表达谱分析
Gene expression profile analysis of Eriocheir sinensis cuticle under acute hypoxia stress
摘要
Abstract
To investigate the gene expression response in the cuticle of Eriocheir sinensis during the post-molt stage un-der acute hypoxia stress,this study utilized Illumina second-generation sequencing technology to detect the gene expression profiles between the control and the acute hypoxia stress treatment,namely(1.0±0.1)mg/L for 1 h.The results showed that compared with the control,a total of 691 differential expressed genes were identified in the acute hypoxia stress treatment.These differentially expressed genes were mainly enriched in physiological activities such as cuticle structural components,chitin binding,and metabolic pathways such as nitrogen metabolism,glyceride metabolism,and glycolysis pathway.Nine significantly differentially expressed genes were screened and verified by real-time fluorescence quantitative PCR.The results of this study preliminarily clarify the mo-lecular response mechanism of cuticle in E.sinensis at the post-molt stage to acute hypoxia stress,laying the founda-tion for further investigation into the effects of hypoxia stress on the molting and growth of E.sinensis.关键词
中华绒螯蟹/急性低氧胁迫/蜕壳后期/表皮基因/转录组分析Key words
Eriocheir sinensis/acute hypoxia stress/post-molt stage/cuticle genes/transcriptome analysis分类
信息技术与安全科学引用本文复制引用
张婷,储俊东,王丹,周军,邓燕飞,张慧敏,许建和,李旭光..急性低氧胁迫下中华绒螯蟹表皮基因表达谱分析[J].江苏农业学报,2025,41(4):724-732,9.基金项目
江苏省种业振兴"揭榜挂帅"项目[JBGS(2021)031、JBGS(2021)125] （2021）
"十四五"国家重点研发计划项目(2022YFD2400700) （2022YFD2400700）
江苏省碳达峰碳中和科技创新专项资金项目(SBE2022350035) （SBE2022350035）
江苏海洋大学研究生科研与实践创新计划项目(KYCX2023-103) （KYCX2023-103）
