昆虫学报2025，Vol.68Issue(4)：450-462,13.DOI:10.16380/j.kcxb.2025.04.007
"菌-生物炭-黑水虻互作"高效生物转化金霉素菌渣体系优化策略
Optimization strategies of the "bacteria-biochar-Hermetia illucens interaction" system for highly efficient bioconversion of chlortetracycline fermentation residue
摘要
Abstract
[Aim]To establish a highly efficient "bacteria-biochar-Hermetia illucens interaction"conversion system and explore the effects of combination strategies,such as substrate ratio,density of H.illucens larvae,biochar,and functional microbes,on the system for converting chlortetracycline fermentation residues of H.illucens.[Methods]H.illucens larvae were reared to the 3rd instar with wheat bran.Then,different mass ratios of chlortetracycline fermentation residues to wheat straw(chlortetracycline fermentation residues∶wheat straw=0∶1,1∶2,1∶4,1∶8,1∶12 and 1∶20),larval densities(larval number∶substrate mass=0.6∶1,0.8∶1,1∶1,2∶1,4∶1,6∶1,8∶1 and 10∶1),biochar addition(0,30,60,90,120,150 and 180 g/kg),functional bacteria(Providencia stuartii TX2,Klebsiella pneumoniae TX1,and Bacillus velezensis EE AM 10B),and mixed addition groups were set up.The growth and development indices of H.illucens larvae(body weight,body length,and body width),substrate conversion indices(substrate consumption rate and substrate conversion rate),and chlortetracycline removal rate in the substrate were measured.[Results]In the optimal "bacteria-biochar-H.illucens interaction" conversion system for chlortetracycline fermentation residues,with a chlortetracycline fermentation residue to straw ratio of 1∶4,a larval density of 1∶4,a biochar addition ratio of 120 g/kg,and the presence of P.stuartii TX2,the larval weight,length and width of H.illucens reached 0.17 g/individual,18.27 mm and 4.83 mm,respectively.Additionally,in the optimal"bacteria-biochar-H.illucens interaction" conversion system,the substrate consumption rate and substrate conversion rate reached 34.04％and 29.50％,respectively,while the chlortetracycline removal rate in the substrate increased to 96.23％.[Conclusion]Through the optimization of the highly efficient "bacteria-biochar-H.illucens interaction" conversion system for antibiotic fermentation residues,two types of organic solid waste were converted into high-value insect protein,while the chlortetracycline removal rate in the substrate was improved.关键词
黑水虻/金霉素菌渣/秸秆/生物炭/功能微生物Key words
Hermetia illucens/chlortetracycline fermentation residue/wheat straw/biochar/functional microbes分类
生物科学引用本文复制引用
雷澳杰,裴亚欣,杨森,孙梦晓,段依琳,张继冉,陈红歌.."菌-生物炭-黑水虻互作"高效生物转化金霉素菌渣体系优化策略[J].昆虫学报,2025,68(4):450-462,13.基金项目
国家重点研发计划项目(2021YFD1700904) （2021YFD1700904）
中国博士后科学基金项目(2023M741055) （2023M741055）
河南省重点研发与推广专项(232102320103,242102321055) （232102320103,242102321055）
河南农业大学高层次人才专项(30501045) （30501045）
