解剖学杂志2025，Vol.48Issue(2)：119-123,177,6.DOI:10.3969/j.issn.1001-1633.2025.02.005
运动诱导的lncRNA MSTRG.19096.5对急性心肌梗死小鼠心室重构的影响
Exercise-induced lncRNA MSTRG.19096.5 affects ventricular remodeling in mice after acute myocardial infarction
岳温恒 1黄鲲 1吴越 1温佳雨 1梁春1
作者信息
- 1. 海军军医大学第二附属医院心血管内科,上海 200001
- 折叠
摘要
Abstract
Objective:To investigate the effects of exercise-induced lncRNA MSTRG.19096.5 on ventricular remodeling in mice with acute myocardial infarction(MI).Methods:Through lncRNA sequencing,we identified the lncRNAs that were highly expressed in myocardial tissue after exercise.The significantly upregulated lncRNA MSTRG.19096.5 was selected for further study.The mice were divided into the sham group,Lv-NC+MI group,Lv-MSTRG.19096.5+MI group,and Lv-sh-RNA-MSTRG.19096.5+MI group.Thirty days later,the effects on post-MI ventricular remodeling were evaluated through cardiac Masson staining(relative length of myocardial scar,myocardial fibrosis ratio),echocardiography[left ventricular ejection fraction(EF),left ventricular fractional shortening(FS)],ELISA[plasma brain natriuretic peptide(BNP)levels],and Western blotting(protein expression levels of left ventricular myocardial fibrosis markers).Results:LncRNA MSTRG.19096.5,which was highly expressed in the mouse myocardial group after exercise,was screened through lncRNA sequencing.Compared to the Lv-NC+MI group,the Lv-MSTRG.19096.5+MI group showed a significant reduction in the relative length of myocardial scar and myocardial fibrosis ratio,a significant increase in EF and FS,a significant decrease in BNP levels,and a significant reduction in the expression levels of myocardial fibrosis markers collagenⅠ,collagenⅢ,α-SMA,and TGF-β1.Conclusion:Exercise-induced lncRNA MSTRG.19096.5 plays a protective role in post-acute myocardial infarction ventricular remodeling by reducing myocardial fibrosis and improving cardiac function.关键词
lncRNA/心肌梗死/心室重构/运动/小鼠Key words
lncRNA/myocardial infarction/ventricular remodeling/exercise/mouse分类
基础医学引用本文复制引用
岳温恒,黄鲲,吴越,温佳雨,梁春..运动诱导的lncRNA MSTRG.19096.5对急性心肌梗死小鼠心室重构的影响[J].解剖学杂志,2025,48(2):119-123,177,6.
