摘要
Abstract
Objective:To investigate the effects and mechanism of emodin in improving glucose and lipid metabolism and inflammatory response in rats with gestational diabetes mellitus(GDM).Methods:The model of gestational diabetes rats was established by combining a high sugar and high fat diet with streptozotocin injection.The model rats were randomly divided into model group(GDM group),low,medium and high dose emodin groups,and metformin group,with an additional normal control group.Histopathological changes and apoptosis of pancreatic cells were detected by H-E and Annexin V-FITC/DAPI staining.Fasting blood glucose(FBG),fasting insulin(FINS),the level of triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),iron ions,and the expression of advanced glycation end products(AGEs)-receptor for advanced glycation end products(RAGE)pathway proteins were detected,respectively.Results:Compared with the GDM group,the pathological injury of the metformin group and the emodin group was improved.The levels of FBG,the apoptosis rate of pancreatic tissue,serum TG,TC,LDL-C,TNF-α,IL-6,IL-1β,MDA,iron ion levels and AGES-AGE signal pathway protein expression were decreased significantly,while serum FINS,the level of SOD and GSH-Px in pancreatic tissue were increased significantly.Conclusion:Emodin can improve glucose-lipid metabolism and systemic inflammatory response in GDM rats,reduce pancreatic tissue apoptosis,and may achieve these effects by modulating the AGEs-RAGE signaling pathway.关键词
大黄素/晚期糖基化终产物/晚期糖基化终产物受体/妊娠糖尿病/大鼠Key words
emodin/advanced glycation end products/receptor of advanced glycation end products/gestational diabetes mellitus/rat分类
医药卫生
