昆虫学报2025，Vol.68Issue(4)：463-476,14.DOI:10.16380/j.kcxb.2025.04.008
毒死蜱对白背飞虱生长发育和繁殖的亚致死效应
Sublethal effects of chlorpyrifos on the growth,development and reproduction of Sogatella furcifera(Hemiptera:Delphacidae)
摘要
Abstract
[Aim]This study aims to explore the sublethal effects of chlorpyrifos on Sogatella furcifera,so as to provide a theoretical basis for the rational use of insecticides to control S.furcifera.[Methods]The 3rd instar nymphs of S.furcifera were treated with LC10(1.5 mg/L)and LC25(2.9 mg/L)of chlorpyrifos using rice stem dipping method.The female adult longevity,adult emergence rate and number of eggs laid per female adult of the F0 generation were detected.The age-stage,two-sex life table of the F1 generation was constructed to analyze the changes in the developmental duration,fecundity and survival rates.The relative expression levels of reproduction-related genes(SfRheb,SfTOR,SfS6K,SfHMGR,SfIPPI,SfFPPS1,SfFPPS2,SfJHAMT,SfFAMeT,SfMet,SfKrh1 and SfVg)in the F0 adult females were determined by RT-qPCR.[Results]The numbers of eggs laid per F0 female adult of S.furcifera in the treatment groups with LC10 and LC25 of chlorpyrifos were significantly reduced,and the adult emergence rate and female adult longevity of the F0 generation in the treatment group with LC25 of chlorpyrifos were shortened significantly,as compared with those in the control group treated with distilled water.The average numbers of eggs laid per F1 female adult in the treatment groups with LC10 and LC25 of chlorpyrifos were 191.4 and 169.9,respectively,both significantly lower than that in the control group(230.6).Exposure of the 3rd instar nymphs of S.furcifera to LC25 of chlorpyrifos significantly shortened the female adult duration of the F,generation and reduced the survival rate of the F1 generation of S.furcifera,compared to the control.Furthermore,exposure of the 3rd instar nymphs of S.furcifera to LC10 and LC25 of chlorpyrifos inhibited the expression of genes(SfFAMeT,SfFPPS1,SfFPPS2 and SfKrh1)related to juvenile hormone(JH)signaling pathways,genes(SfRheb and SfTOR)related to TOR signaling pathways,and SfVg in female adults of the F0 generation.[Conclusion]Sublethal concentrations of chlorpyrifos stress inhibited the growth,development,survival rate and fecundity of S.furcifera,thereby suppressing the population growth of subsequent generations to a certain extent.Furthermore,sublethal concentrations of chlorpyrifos also down-regulated the expression of genes related to the TOR and JH signaling pathways.These findings provide a theoretical basis for the scientific control of S.furcifera in the field and lay a foundation for understanding the molecular mechanisms by which insecticides regulate insect reproduction.关键词
白背飞虱/毒死蜱/亚致死效应/年龄-龄期两性生命表/繁殖力/生长发育Key words
Sogatella furcifera/chlorpyrifos/sublethal effect/age-stage,two-sex life table/fecundity/growth and development分类
生物科学引用本文复制引用
任书瑾,刘露遥,伊丽姆努尔·玉山,桂航,张世燕,何树林,章冰川,乔梁,周操..毒死蜱对白背飞虱生长发育和繁殖的亚致死效应[J].昆虫学报,2025,68(4):463-476,14.基金项目
重庆市博士"直通车"科研项目(CSTB2022BSXM-JCX0067) （CSTB2022BSXM-JCX0067）
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0235) （cstc2021jcyj-msxmX0235）
重庆市教委科学技术研究项目(KJQN202200533,KJQN202200532) （KJQN202200533,KJQN202200532）
