摘要
Abstract
Objective:To investigate the effect of tea polyphenols(TP)on the inflammatory response in keratinocytes from condyloma acuminatum(CA)by regulating the Hippo/Yes-associated protein 1(YAP1)pathway.Methods:Hacat cells with stable expression of HPV16E6(HPV-Hacat)were cultured and were divided into control group,L-TP group(50 μg/mL TP),M-TP group(100 μg/mL TP),H-TP group(200 μg/mL TP),H-TP+TDI-011536 group(200 μg/mL TP+3 μmol/L Hippo pathway activator TDI-011536).MTT and EdU methods were applied to determine cell proliferation.Cell scratch assay and Transwell test were adopted to determine the migration and invasion of cells,respectively.ELISA method was employed to determine the contents of TNF-α and IL-6.Western blotting was utilized to determine the expression of Hippo/YAP1 pathway proteins and migration proteins in cells.Results:The survival rate of HPV-Hacat cells gradually decreased with the increase in TP concentration and showed a dose-dependent relationship.Compared with the control group,the proliferation,migration,invasion,TNF-α,IL-6 levels,YAP1,and matrix metalloproteinase(MMP)-2 and MMP-9 protein expression of HPV-Hacat cells in the L-TP group,M-TP group,and H-TP group were all reduced,with more pronounced effects observed at higher doses of TP.Compared with the H-TP group,the H-TP+TDI-011536 group showed an increase in HPV-Hacat proliferation,migration,invasion,TNF-α,IL-6 levels,YAP1,MMP-2,and MMP-9 protein expression.Conclusion:TP can effectively inhibit the proliferation,invasion,migration,and inflammatory response of HPV-Hacat cell,and its mechanism may be related to the inhibition of Hippo/YAP1 signaling pathway activation.关键词
茶多酚/尖锐湿疣/角质形成细胞/炎症反应/Hippo/Yes关联蛋白1Key words
tea polyphenols/condyloma acuminatum/keratinocytes/inflammatory response/Hippo/Yes-associated protein 1分类
医药卫生
