解剖学杂志2025，Vol.48Issue(2)：143-148,181,7.DOI:10.3969/j.issn.1001-1633.2025.02.010
丝氨酸蛋白酶抑制剂E2通过调节上皮间质转化促进黑色素瘤细胞增殖和侵袭
Serine protease inhibitor E2 promotes melanoma cell proliferation and invasion by modulating epithelial-mesenchymal transition
摘要
Abstract
Objective:To investigate the effect and mechanism of serine protease inhibitor E2(SERPINE2)on the proliferation and invasion of melanoma cells.Methods:The expression of SERPINE2 in nevi,melanoma tumors and metastases was analyzed through TCGA database and immunohistochemical staining of clinical samples.After the knockdown of SERPINE2 expression in melanoma cell A375,SERPINE2 mRNA and protein expression were detected by RT-qPCR and Western blotting.CCK8 assay was used to detect cell proliferation,Transwell assay to detect cell invasion,and Western blotting to measure E-cadherin(CDH1),N-cadherin(CDH2),SNAI1,matrix metalloprotease(MMP)3 and MMP9 protein expression.Results:TCGA database analysis revealed that SERPINE2 expression was higher in melanoma compared with normal tissues.Immunohistochemical staining analysis confirmed that SERPINE2 was highly expressed in primary and metastatic melanoma.Knockdown of SERPINE2 significantly inhibited the proliferation and invasion of A375 cells and affected the expression of EMT pathway molecules.Specifically,the expression of CDH1 was significantly increased,while the expression of CDH2,SNAI1,MMP3 and MMP9 were significantly decreased.Conclusion:SERPINE2 promotes the proliferation and invasion of melanoma cells by regulating the expression of epithelial-mesenchymal transition-related molecules and targeting SERPINE2 and may be a promising strategy for inhibiting melanoma tumor progression.关键词
丝氨酸蛋白酶抑制剂E2/黑色素瘤/增殖/侵袭/上皮间质转化Key words
serine protease inhibitor E2/melanoma/proliferation/invasion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
陈珂欣,汪子愉,吴敏靓,王宇翀,熊俊,薛春雨..丝氨酸蛋白酶抑制剂E2通过调节上皮间质转化促进黑色素瘤细胞增殖和侵袭[J].解剖学杂志,2025,48(2):143-148,181,7.基金项目
军队重点专科项目 （）
