昆虫学报2025，Vol.68Issue(4)：487-498,12.DOI:10.16380/j.kcxb.2025.04.010
氟氯氰菊酯对中华按蚊发育和繁殖的亚致死效应
Sublethal effects of cyfluthrin on the development and reproduction of Anopheles sinensis(Diptera:Culicidae)
摘要
Abstract
[Aim]Cyfluthrin,a novel pyrethroid insecticide,is extensively used in the control of agricultural and sanitary pests.This study aims to explore the sublethal effects of cyfluthrin on Anopheles sinensis and provide a theoretical foundation for the rational use of cyfluthrin in the control of An.sinensis.[Methods]The 4th instar larvae of An.sinensis were treated with cyfluthrin for 24 h using the larval immersion method recommended by the World Health Organization(WHO).The LC10 and LC30 values of cyfluthrin against the 4th instar larvae of An.sinensis were determined.The 4th instar larvae of An.sinensis were exposed to LC10 and LC30 of cyfluthrin for 24 h by the larval immersion method.The female and male adult longevity and number of eggs laid per female of the F0 generation were recorded.The eggs laid by the female adults of the F0 generation were collected and used as the F1 generation insects.The hatching rate,duration of each developmental stage,emergence rate,adult pre-oviposition period,total pre-oviposition period,and number of eggs laid per female of the F1 generation were counted.Life table was constructed to predict the population dynamics of An.sinensis in each treatment.[Results]The LC10 and LC30 values of cyfluthrin against the 4th instar larvae of An.sinensis after 24-h exposure were 1.223 × 10-3 and 4.454 × 10-3 mg/L,respectively.In comparison to the control(exposure to dechlorinated tap water),exposure of the 4 th instar larvae of An.sinensis to LC10 and LC30 of cyfluthrin significantly reduced the male adult longevity and the number of eggs laid per female of the F0 generation,and exposure of the 4th instar larvae of An.sinensis to LC30 of cyfluthrin also significantly shortened the female adult longevity of the F0 generation.fx7 curves showed that exposure of the 4th instar larvae of An.sinensis to LC10 and LC30 of cyfluthrin reduced the numbers of oviposition days and eggs laid by female adults of the F,generation,and decreased the peak values of lxmx,mx and vxj in the F1 generation,compared with the control group.Exposure of the 4th instar larvae of An.sinensis to LC10 of cyfluthrin delayed the peak of mx in the F,generation,while exposure of the 4th instar larvae of An.sinensis to LC30 of cyfluthrin advanced the peak of mx in the F1 generation.The sxj,lx and exj curves showed that exposure of the 4th instar larvae of An.sinensis to LC10 and LC30 of cyfluthrin had a beneficial effect on the survival rate and life expectancy of the F1 generation.In the treatment group with LC10 of cyfluthrin,the pupal duration and pre-adult duration of the F1 females and the egg duration of the males were significantly prolonged,while the longevity and total developmental duration of the F1 males were significantly shortened,as compared to those in the control group.The stress of cyfluthrin at LC10 and LC30 to the 4th instar larvae of An.sinensis significantly increased pre-adult survival rate of the F1 generation,yet significantly reduced the number of eggs laid per F1 female.Furthermore,exposure of the 4th instar larvae of An.sinensis to LC10 of cyfluthrin significantly prolonged the mean generation time(T)of the F1 generation;in contrast,exposure of the 4th instar larvae of An.sinensis to LC30 of cyfluthrin significantly shortened the mean generation time of the F1 generation.[Conclusion]The stress of cyfluthrin at the sublethal concentrations(LC10 and LC30)significantly inhibited the reproductive capacity of the F1 generation of An.sinensis,but increased the survival rate and life expectancy of the F1 generation,resulting in no significant impact on the F1 population size.These findings provide a theoretical foundation for the rational use of cyfluthrin in the control of An.sinensis.关键词
中华按蚊/拟除虫菊酯/氟氯氰菊酯/亚致死效应/年龄-龄期两性生命表Key words
Anopheles sinensis/pyrethroid/cyfluthrin/sublethal effect/age-stage,two-sex life table分类
生物学引用本文复制引用
张粒,张世燕,张煬,王烟雨,王乙茗,何树林,乔梁,陈斌,周操..氟氯氰菊酯对中华按蚊发育和繁殖的亚致死效应[J].昆虫学报,2025,68(4):487-498,12.基金项目
重庆市教委科学技术研究项目(KJQN202200533) （KJQN202200533）
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0235) （cstc2021jcyj-msxmX0235）
