摘要
Abstract
Objective:To investigate the impacts of long non-coding RNA carbonyl reductase 3-AS1(CBR3-AS1)on the malignant biological behavior of colon cancer cells by regulating microRNA-145-5p(miR-145-5p)/S-phase kinase-associated protein 1(SKP1)axis.Methods:Colon cancer cell line SW480 was divided into five groups:control group,si-NC group,si-CBR3-AS1 group,si-CBR3-AS1+anti miR-NC group,and si-CBR3-AS1+anti miR-145-5p group.RT-qPCR was applied to detect the expression of CBR3-AS1,miR-145-5p,and SKP1 mRNA in cells.CCK-8 assay was applied to detect cell proliferation.Transwell assay was utilized to detect cell migration and invasion abilities,and the flow cytometry was employed to detect the level of cell apoptosis.Western blotting was adopted to detect the expression levels of SKP1,B-cell lymphoma protein 2(Bcl-2)-associated X(Bax),and B-cell lymphoma protein 2(Bcl-2)in cancer cells.Dual-luciferase reporter assay was applied to verify the interaction between miR-145-5p and CBR3-AS1,as well as between miR-145-5p and SKP1 mRNA.Results:Compared with the control group and si-NC group,the miR-145-5p,apoptosis rate,and the expression of Bax protein in SW180 cells in the si-CBR3-AS1 group were increased,while the expression of SKP1 mRNA,CBR3-AS1,OD450 values(24,48,72 h),numbers of migrating and invading cells,and the expression of Bcl-2 protein were decreased.Down-regulation of miR-145-5p weakened the inhibitory effects of low-expressed CBR3-AS1 on the proliferation,migration,and invasion of SW480 cells,and also inhibited cell apoptosis.CBR3-AS1 targeted and negatively regulated the miR-145-5p expression,while miR-145-5p targeted and negatively regulated the SKP1 mRNA expression.Conclusion:The CBR3-AS1/miR-145-5p/SKP1 axis can inhibit proliferation,migration,and invasion of SW480 cells,and promote apoptosis in SW480 cells.关键词
长链非编码RNA羰基还原酶3反义RNA1/miR-145-5p/S期激酶关联蛋白1/结肠癌/恶性生物学行为Key words
carbonyl reductase 3-AS1/miR-145-5p/S-phase kinase-associated protein 1/colon cancer/malignant biological behavior分类
医药卫生
