昆虫学报2025，Vol.68Issue(4)：499-508,10.DOI:10.16380/j.kcxb.2025.04.011
韭菜迟眼蕈蚊的抗药性监测及对灭蝇胺的抗性风险和亚致死效应分析
Monitoring of insecticide resistance in Bradysia odoriphaga(Diptera:Sciaridae),and analysis of resistance risk and sublethal effect of cyromazine
摘要
Abstract
[Aim]To ascertain the resistance status of Bradysia odoriphaga to ten insecticides,the potential risk of resistance of B.odoriphaga to cyromazine and the sublethal effects of cyromazine on B.odoriphaga.[Methods]We determined the toxicity of 10 insecticides of 5 categories(neonicotinoids,pyrethroids,pyrroles,insect growth regulators and organophosphates)to the 2nd instar larvae of B.odoriphaga from four regions including Puyang City in Henan Province,Bozhou City in Anhui Province,Tangshan City in Hebei Province,and Jining City in Shandong Province with the stomach-contact combination toxicity method.We observed the effects of sublethal concentrations(LC15 and LC30)of cyromazine on the larval duration,pupation rate,emergence rate and number of eggs laid per female of B.odoriphaga,and assessed the risk of resistance to cyromazine.We calculated the realized heritability(h2)of resistance of B.odoriphaga using Tabashnik's method for threshold trait analysis and predicted the resistance development rates under different selection pressures based on the data of selection.[Results]Monitoring data demonstrated that the field populations of B.odoriphaga were susceptible or exhibited low-level resistance to cyromazine,hexaflumuron,chlorfluazuron and pyriproxyfen,and showed low to moderate levels of resistance to phoxim.All the monitored populations of B.odoriphaga showed low-level resistance to beta-cypermethrin.All field populations of B.odoriphaga were susceptible or developed low to moderate levels of resistance to imidacloprid,clothianidin and thiamethoxam.It would take 25.2,20.7,17.2,14.4 and 11.4 generations,respectively,to develop 10-fold resistance to cyromazine in B.odoriphaga,under different selection pressures(mortality rates of 50％,60％,70％,80％and 90％,respectively)when the realized heritability(h2)of resistance was 0.073.The emergence rates and numbers of eggs laid per female of F0 and F1 generations of B.odoriphaga were significantly reduced after exposure of the 2nd instar larvae to LC15 and LC30 of cyromazine.[Conclusion]B.odoriphaga has low resistance risk to cyromazine.Cyromazine treatment at sublethal concentrations exhibits significant inhibitory effects on the pupation rate,emergence rate,and number of eggs laid per female of B.odoriphaga.关键词
韭菜迟眼蕈蚊/抗药性监测/灭蝇胺/抗性风险/适合度代价Key words
Bradysia odoriphaga/insecticide resistance monitoring/cyromazine/resistance risk/fitness cost分类
生物科学引用本文复制引用
刘佳乐,苏少聪,王洪涛,刘保友,柴宏飞,高聪芬,吴顺凡..韭菜迟眼蕈蚊的抗药性监测及对灭蝇胺的抗性风险和亚致死效应分析[J].昆虫学报,2025,68(4):499-508,10.基金项目
三亚南京农业大学研究院引导资金项目(NAUSY-MS15) （NAUSY-MS15）
中央高校基本科研业务费专项资金(KJJQ2024016) （KJJQ2024016）
国家自然科学基金项目(32022011) （32022011）
