兰州大学学报（医学版）2025，Vol.51Issue(4)：40-47,58,9.DOI:10.13885/j.issn.1000-2812.2025.04.006
氨甲环酸温敏水凝胶的制备及体外凝血活性评价
Preparation and evaluation of in vitro coagulation activity of tranexamic acid thermosensitive hydrogel
摘要
Abstract
Objective To prepare tranexamic acid(TXA)thermosensitive hydrogel and investigate its drug release and coagulation activity in vitro.Methods TXA thermosensitive hydrogel was prepared by cold solu-tion method using poloxamer 407(P407)and poloxamer 188(P188)as the matrix,and its preparation process optimized through orthogonal test design.The gel performance was systematically evaluated using gelling temperature,gelling time,Fourier transform infrared(FTIR)and in vitro release effect.The in vitro coagula-tion activity of TXA thermosensitive hydrogel was assessed by measuring activated partial thromboplastin time,prothrombin time,and thrombin time.Results The optimal preparation process conditions of TXA ther-mosensitive hydrogel were 20 g/mL P407+3 g/mL P188+3 g/mL TXA,its gelling temperature was about 35°C,gelling time was 166 s,the FTIR analysis confirmed the successful preparation of the P407/P188 thermosensi-tive hydrogel loaded with TXA in this study.The cumulative release rate reached 95.52%in 8 h,and the re-lease process was in accordance with the Higuchi model.The low,medium,and high-dose TXA thermosensi-tive hydrogel could all significantly shorten activated partial thromboplastin time and thrombin time,while the medium and high-dose groups could significantly shorten prothrombin time.Conclusion The TXA ther-mosensitive hydrogel exhibited excellent sustained-release effects and hemostatic functions,and has potential application prospects in controlling bleeding and promoting wound healing.关键词
氨甲环酸/温敏水凝胶/工艺优化/缓释/凝血活性Key words
tranexamic acid/thermosensitive hydrogel/process optimization/sustained release/coagulation activity分类
药学引用本文复制引用
范军红,李少华,郭玉琴,张皓岚,张雅欣,曹宇杰,余兰..氨甲环酸温敏水凝胶的制备及体外凝血活性评价[J].兰州大学学报（医学版）,2025,51(4):40-47,58,9.基金项目
国家自然科学基金资助项目(51501080) （51501080）
兰州市科技计划资助项目(2023-1-58) （2023-1-58）
甘肃省高校研究生"创新之星"资助项目(2025CXZX-197) （2025CXZX-197）
