检验医学与临床2025，Vol.22Issue(10)：1328-1333,6.DOI:10.3969/j.issn.1672-9455.2025.10.007
血清miR-425-5p和miR-144-5p水平对肺癌患者早期诊断及预后的临床价值
Clinical value of serum miR-425-5p and miR-144-5p levels in early diagnosis and prognosis of lung cancer patients
摘要
Abstract
Objective To explore the clinical value of serum microRNA(miR)-425-5p and miR-144-5p lev-els in early diagnosis and prognosis of lung cancer patients.Methods A total of 107 patients with stage Ⅰ—Ⅱ lung cancer diagnosed in Affiliated Jiangyin People's Hospital of Nantong University from March 2019 to August 2020 were selected as the early lung cancer group.In addition,107 patients with benign pulmonary dis-eases who underwent physical examination in Affiliated Jiangyin People's Hospital of Nantong University dur-ing the same period were selected as the control group.The levels of miR-425-5p and miR-144-5p in early lung cancer group and control group were compared.Clinical data of all subjects were collected.Multivariate Logis-tic regression was used to analyze the influencing factors of early lung cancer.The receiver operating charac-teristic(ROC)curve was used to analyze the diagnostic value of miR-425-5p and miR-144-5p in patients with early lung cancer.Kaplan-Meier survival curve was used to analyze the relationship between the levels of miR-425-5p and miR-144-5p and the death of patients with early lung cancer.Results The levels of carcinoembry-onic antigen(CEA),neuron-specific enolase(NSE),cytokeratin 19 fragment(CYRFA21-1)and miR-425-5p in the early lung cancer group were higher than those in the control group,and the level of miR-144-5p was lower than that in the control group,and the differences were statistically significant(P＜0.05).Multivariate Logistic regression analysis showed that serum CEA,NSE,CYRFA21-1,miR-425-5p and miR-144-5p were in-fluencing factors for the occurrence of early lung cancer(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of serum miR-425-5p,miR-144-5p and their combination in the diagnosis of early lung cancer were 0.859,0.853 and 0.911 respectively,the AUC of the combined diagnosis of the two was greater than that of the single diagnosis(Zcombined with miR-425-5p=2.053,Zcombined with miR-144-5p=2.504,P＜0.05).The sensitiv-ity and specificity of the combined diagnosis of the two were 71.03％and 96.26％respectively.The 3-year fol-low-up rate was 100％,and no one was lost to follow-up.Kaplan-Meier survival curve analysis showed that the 3-year survival rate of early lung cancer patients with low miR-425-5p expression(94.44％,51/54)was higher than that of early lung cancer patients with high miR-425-5p expression(81.13％,43/53,x2=4.441,P＜0.05).The 3-year survival rate of early lung cancer patients with high miR-144-5p expression(96.30％,52/54)was higher than that of low miR-144-5p expression(79.25％,42/53,x2=7.286,P＜0.05).There was a significant difference in the survival curve between early lung cancer patients with low miR-425-5p expression and high miR-425-5p expression(Log-rank x2=4.551,P=0.033).There was a significant difference in the survival curve between early lung cancer patients with low miR-144-5p expression and the high miR-144-5p expression(Log-rank x2=4.755,P=0.029).Conclusion The level of miR-425-5p is increased and the level of miR-144-5p is decreased in patients with early lung cancer.The levels of miR-425-5p and miR-144-5p are closely related to death of patients with early lung cancer.The combined detection of miR-425-5p and miR-144-5p has good clinical value for early diagnosis and prognosis of lung cancer.关键词
微小RNA-425-5p/微小RNA-144-5p/肺癌/早期诊断/预后Key words
microRNA-425-5p/microRNA-144-5p/lung cancer/early diagnosis/prognosis分类
临床医学引用本文复制引用
许群,张金,曹丹丹,陶俊,郁芳芳..血清miR-425-5p和miR-144-5p水平对肺癌患者早期诊断及预后的临床价值[J].检验医学与临床,2025,22(10):1328-1333,6.基金项目
江苏省重点研发计划(社会发展)专项资金项目(BE2021783). （社会发展）
