检验医学与临床2025，Vol.22Issue(10)：1355-1360,6.DOI:10.3969/j.issn.1672-9455.2025.10.012
特发性肺纤维化患者血清LncRNA MEG3、miR-145-5p水平与病情严重程度、肺功能、TLR4、MyD88、NF-κB的关系
Relationship between serum LncRNA MEG3 and miR-145-5p levels and disease severity,lung function,TLR4,MyD88,and NF-κB in patients with idiopathic pulmonary fibrosis
摘要
Abstract
Objective To investigate the relationship between serum long non-coding RNA maternally ex-pressed gene 3(LncRNA MEG3),microRNA-145-5p(miR-145-5p)levels and disease severity,lung function,Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88)and nuclear factor κB(NF-κB)in pa-tients with idiopathic pulmonary fibrosis(IPF).Methods A total of 98 IPF patients admitted to the hospital from February 2020 to January 2023 were randomly selected as the study group,and 100 healthy volunteers who underwent physical examination in the hospital during the same period were randomly selected as the control group.The serum levels of LncRNA MEG3 and miR-145-5p were compared between the two groups.Patients with IPF were divided into stable group and acute exacerbation group according to chest high-resolu-tion computed tomography(HRCT)score.The serum levels of LncRNA MEG3,miR-145-5p,TLR4,MyD88,and NF-κB mRNA,forced expiratory volume in one second as a percentage of predicted value(FEV1％),car-bon monoxide diffusion capacity as a percentage of predicted value(DLCO％)and HRCT score were com-pared between the two groups.Spearman correlation was used to analyze the correlation between serum levels of LncRNA MEG3 and miR-145-5p and the severity of IPF,lung function,and TLR4/MyD88/NF-κB signaling pathway in patients with IPF.Receiver operating characteristic(ROC)curve was used to analyze the diagnos-tic value of serum LncRNA MEG3,miR-1 45-5p alone and combined detection for IPF.Results The serum level of LncRNA MEG3 in the study group was lower than that in the control group,and the serum level of miR-145-5p was higher than that in the control group,and the differences were statistically significant(P＜0.05).Twenty-four patients were included in the acute exacerbation group and 74 patients were included in the stable group.The levels of FEV1％,DLCO％,and LncRNA MEG3 in the acute exacerbation group were lower than those in the stable group,and the HRCT score,serum TLR4,MyD88,NF-κB mRNA,and miR-145-5p levels were higher than those in the stable group,and the differences were statistically significant(P＜0.05).Spearman correlation analysis showed that serum LncRNA MEG3 level was negatively correlated with HRCT score,TLR4,MyD88 and NF-κB mRNA levels(P＜0.05),and positively correlated with FEV1％and DLCO％(P＜0.05).The level of serum miR-145-5p was positively correlated with HRCT score and the mR-NA levels of TLR4,MyD88 and NF-κB(P＜0.05),and negatively correlated with FEV1％and DLCO％(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of serum LncRNA MEG3 and miR-145-5p alone and in combination to diagnose the occurrence of IPF were 0.863,0.867 and 0.934 respectively.The AUC of combined detection in diagnosing IPF was greater than that of each index alone(Z=2.162,2.031,P＜0.05).Conclusion Serum LncRNA MEG3 level is decreased and miR-145-5p level is increased in IPF patients,which are closely related to the severity of IPF,lung function and TLR4/MyD88/NF-κB signa-ling pathway gene expression.Combined detection of lncrna MEG3 and miR-145-5p has a high diagnostic value for the occurrence of IPF.关键词
特发性肺纤维化/长链非编码核糖核酸母系表达基因3/微小核糖核酸-145-5p/病情严重程度/肺功能/Toll样受体4/髓样分化因子88/核因子-κB信号通路Key words
idiopathic pulmonary fibrosis/long non-coding RNA maternally expressed gene 3/mi-croRNA-145-5p/severity of disease/pulmonary function/Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor κB signaling pathway分类
医药卫生引用本文复制引用
张翠翠,李向东,蔡仁萍,韩涛..特发性肺纤维化患者血清LncRNA MEG3、miR-145-5p水平与病情严重程度、肺功能、TLR4、MyD88、NF-κB的关系[J].检验医学与临床,2025,22(10):1355-1360,6.基金项目
山东省医药卫生科技发展计划(2018WS444) （2018WS444）
山东省济南市卫生健康委员会科技计划(2020-4-150、2021-2-63). （2020-4-150、2021-2-63）
