Abstract
Objective To investigate the levels and clinical significance of serum long non-coding RNA(ln-cRNA)X-inactive specific transcript(XIST)and lncRNA myocardial infarction associated transcript(MIAT)in the patients with diabetic nephropathy(DN).Methods A total of 100 patients with simple T2DM(T2DM group)and 112 patients with DN(DN group)who visited the hospital from April 2020 to April 2023 were se-lected as the research objects,and 100 healthy subjects who underwent physical examination at the physical examination center of the hospital during the same period were selected as the control group.The levels of ser-um lncRNA XIST and lncRNA MIAT in the three groups were compared.The clinical data of the T2DM group and the DN group were collected.Pearson correlation was used to analyze the correlation between the levels of serum lncRNA XIST and lncRNA MIAT and inflammatory factors[interleukin-1β(IL-1β),interleu-kin-6(IL-6),tumor necrosis factor(TNF-α)[,and renal fibrosis indicators[urinary hyaluronic acid(HA),u-rinary laminin(LN),typeⅣ collagen(CⅣ)]in patients with DN;multivariate Logistic regression was used to analyze the factors affecting the occurrence of DN;the receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum lncRNA XIST,lncRNA MIAT and other influencing factors for DN.Results The serum lncRNA XIST,lncRNA MIAT,IL-1β,IL-6,TNF-α,HA,LN and CⅣ levels in the T2DM group and the DN group were higher than those in the control group(P＜0.05);the levels of serum lncRNA XIST,lncRNA MIAT,IL-1β,IL-6,TNF-α,HA,LN and CⅣ in the DN group were higher than those in the T2DM group(P＜0.05).The results of Pearson correlation analysis showed that the serum lncRNA XIST and lncRNA MIAT levels in patients with DN were positively correlated with IL-1β,IL-6,TNF-α,and HA,LN and CⅣ levels(P＜0.05).The course of DM,urine albumin/creatinine ratio(ACR),hypersensitive C-reactive protein(hs-CRP),glycated hemoglobin(HbA1c),blood urea nitrogen(BUN),serum creatinine(Scr),and cystatin C(Cys-C)levels in the DN group were higher than those in the T2DM group(P＜0.05).The results of multivariate Logistic regression analysis showed that elevated levels of HbA1c,hs-CRP,ln-cRNA XIST and lncRNA MIAT were independent risk factors for the occurrence of DN(P＜0.05).The re-sults of ROC curve analysis showed that the AUCs of single serum lncRNA XIST,lncRNA MIAT,HbA1c,hs-CRP and the combined diagnosis of the four items for DN were 0.668,0.689,0.731,0.656 and 0.917,re-spectively.The AUC of the combined diagnosis was higher than that of single diagnosis of serum lncRNA XIST,lncRNA MIAT,HbA1c and hs-CRP(Z=6.141,7.468,5.730,6.723,P＜0.001).Conclusion The el-evated levels of serum lncRNA XIST and lncRNA MIAT in DN patients are the independent risk factors for the occurrence of DN.The combined diagnosis of serum lncRNA XIST,lncRNA MIAT,HbA1c,and hs-CRP has a high value for DN.关键词
糖尿病肾病/长链非编码RNA/X染色体失活特异转录本/心肌梗死相关转录本/诊断Key words
diabetic nephropathy/long non-coding RNA/X chromosome inactivation specific tran-script/myocardial infarction associated transcript/diagnosis分类
医药卫生
