老年医学与保健2025，Vol.31Issue(2)：443-448,453,7.
基于蛋白质组学研究淫羊藿次苷Ⅱ对巨噬细胞的免疫调控作用
A study on immunoregulatory effects of icariside Ⅱ on macrophages based on proteomics
摘要
Abstract
Objective To investigate the immunoregulatory effects of icariside Ⅱ(ICA Ⅱ)on macrophages polariza-tion and its molecular mechanism based on proteomics technology.Methods RAW264.7 mouse peritoneal macrophages were polarized into M1-type[induced by lipopolysaccharide(LPS)]or M2-type[induced by interleukin-4(IL-4)],respectively.Then they were treated with 10 μmol/L ICA Ⅱ.Subsequently,Label-free quantitative proteomics(LFQ)analysis was conduc-ted.Results In M1 macrophages treated with ICA Ⅱ,809 differentially expressed proteins were identified,of which 443 were up-regulated and 366 were down-regulated.They were enriched in signaling pathways such as metabolic pathway,lysosome,autophagy,and oxidative phosphorylation.In M2 macrophages treated with ICA Ⅱ,654 differentially expressed proteins were identified,of which 324 were up-regulated and 330 were down-regulated.They were enriched in fatty acid metabolism,PPAR-γ,VEGF signaling pathway,oxidative phosphorylation,ferroptosis.Conclusion ICA Ⅱ exerts immunomodulatory effects by regulating fatty acid metabolism,PPAR-γ and VEGF signaling pathways and mediating M1/M2 polarization of macrophages.This study provides a reference basis for the application of ICA Ⅱ in controlling chronic inflammation and immunosenescence of age-related diseases.关键词
巨噬细胞/慢性炎症/淫羊藿次苷Ⅱ/免疫调节/蛋白质组学技术/信号通路Key words
macrophages/chronic inflammation/icariside Ⅱ/immune regulation/proteomics technology/signaling pathway引用本文复制引用
陈蒙蒙,王纳,董竞成..基于蛋白质组学研究淫羊藿次苷Ⅱ对巨噬细胞的免疫调控作用[J].老年医学与保健,2025,31(2):443-448,453,7.基金项目
国家自然科学基金(82174170) （82174170）
国家自然科学基金青年项目(8220518) （8220518）
