老年医学与保健2025，Vol.31Issue(2)：449-453,5.
淫羊藿苷干预支气管哮喘小鼠自噬的机制研究
A study on mechanism of icariin in intervention of autophagy in mice with bronchial asthma
摘要
Abstract
Objective To observe the effects of icariin on autophagy and the MAPK/Erk1/2/mTOR pathway in asth-matic mice.Methods 40 SPF-grade mice were divided into blank control group(group A),asthma model group(group B),low-dose icariin intervention group(group C)and high-dose icariin intervention group(group D).Pulmonary function instru-ment was used to detect airway resistance(RL)and dynamic lung compliance(Cdyn)of mice.ELISA was employed to meas-ure the levels of inflammatory cytokines(IL-6 and TNF-α).Pathological changes in lung tissue were observed via HE stai-ning.Transmission electron microscope was used to observe the ultrastructure of bronchial epithelial cells.Western blot was used to determine the expression of autophagy-related proteins and signaling pathway proteins in lung tissue.Results In the asthma model group,the Cdyn decreased and RL increased(P＜0.01);the levels of serum IL-6 and TNF-α increased(P＜0.05);the airway wall thickened and the infiltration of inflammatory cells increased;autophagosomes accumulated in the epi-thelial cells of lung tissue,and the nuclei shrunken;the expression levels of LC3 Ⅱ and Beclin-1 increased,while P62 de-creased(P＜0.001);the expression levels of p-mTOR,p-P38 MAPK and p-ERK1/2 decreased(P＜0.01).After interven-tion with icariin,airway hyperresponsiveness decreased(P＜0.05)and serum inflammatory cytokines decreased(P＜0.05)in asthmatic mice;the infiltration of airway inflammatory cells decreased,the number of autophagosomes decreased;the expres-sion levels of LC3 Ⅱ and Beclin-1 decreased(P＜0.001),and P62,p-mTOR,p-p38 MAPK,and p-ERK1/2 increased(P＜0.001).Conclusion Icariin can improve airway hyperresponsiveness and inflammation in asthmatic mice,and inhibit the au-tophagy of lung epithelial cells induced by asthma.关键词
支气管哮喘/淫羊藿苷/自噬/MAPK/Erk1/2/mTOR信号通路Key words
bronchial asthma/icariin/autophagy/MAPK/Erk1/2/mTOR signaling pathway引用本文复制引用
宗雨婷,刘家齐,董竞成..淫羊藿苷干预支气管哮喘小鼠自噬的机制研究[J].老年医学与保健,2025,31(2):449-453,5.基金项目
国家自然科学基金青年项目(82104630) （82104630）
