林业科学2025，Vol.61Issue(5)：46-60,15.DOI:10.11707/j.1001-7488.LYKX20240239
模拟氮沉降对中亚热带桉树人工林土壤微生物群落结构及酶活性的影响
Effects of Simulated Nitrogen Deposition on Soil Microbial Community Structure and Enzyme Activities in Eucalyptus Plantations in Mid-subtropical Region
摘要
Abstract
[Objective]The central subtropical region is facing a challenge of increasing nitrogen deposition.Soil microorganisms are the link for maintaining terrestrial ecosystems and sensitive to global change.This study aims to explore the response of soil microorganisms and enzyme activities to the increased nitrogen deposition in Eucalyptus plantations in mid-subtropical region,so as to provide scientific basis for further research on the impact of nitrogen deposition on Eucalyptus plantation ecosystems under the background of global change.[Method]A simulated nitrogen deposition experiment was established in Eucalyptus plantations in 2018,and four treatments were set up:CK(0 kg·hm-2a-1),low nitrogen(LN,50 kg·hm-2a-1),medium nitrogen(MN,100 kg·hm-2a-1)and high nitrogen(HN,150 kg·hm-2a-1).After five years of simulated nitrogen deposition treatment,soil physical and chemical properties,microbial community structure,microbial diversity and soil enzyme activities were analyzed.[Result]1)Compared with the control,nitrogen deposition significantly increased soil cation exchange capacity,and the content of clay,soil organic carbon,total nitrogen,nitrate nitrogen,dissolved organic nitrogen,and microbial biomass nitrogen.As the level of nitrogen deposition increased,the content of total phosphorus,microbial biomass phosphorus,ammonium nitrogen and sand showed a trend of first increase and then decrease.The silt content showed a trend of first decrease and then slight increase,which was lower than that of the control.2)Nitrogen deposition increased the total phospholipid fatty acids(PLFAs)of soil microorganisms.Low and medium nitrogen deposition treatments increased the content of soil bacteria,fungi,Gram-positive bacteria,actinomycetes,arbuscular mycorrhizal fungi and Gram-negative bacteria,and promoted the activities of soil β-glucosidase,cellulase,catalase,polyphenol oxidase,β-N-acetylglucosaminidase,urease and acid phosphatase.However,all the parameters tended to decrease under high nitrogen deposition.3)The results of correlation and redundancy analysis showed that soil cation exchange capacity,microbial biomass nitrogen,silt,total nitrogen,dissolved organic nitrogen and total phosphorus contents were the key environmental factors affecting the changes of microbial community structure and soil enzyme activities.[Conclusion]The short-term simulated nitrogen deposition of five years has improved the soil physico-chemical properties in Eucalyptus plantations,changed the soil microbial community structure and increased soil enzyme activities.Low and medium nitrogen deposition levels have a promoting effect on soil physicochemical properties,microbial community structure and soil enzyme activities,while high nitrogen deposition shows a certain inhibitory effect.关键词
氮沉降/微生物群落结构/磷脂脂肪酸/酶活性/桉树人工林Key words
nitrogen deposition/microbial community structure/phospholipid fatty acid(PLFA)/enzyme activities/Eucalyptus plantation分类
农业科技引用本文复制引用
孙英杰,吕仕洪,褚俊智,张德楠,沈育伊,徐广平,曹杨,黄科朝,陈运霜,毛馨月,滕秋梅..模拟氮沉降对中亚热带桉树人工林土壤微生物群落结构及酶活性的影响[J].林业科学,2025,61(5):46-60,15.基金项目
国家自然科学基金项目(42267007,31760162) （42267007,31760162）
广西自然科学基金项目(2018GXNSFAA050069) （2018GXNSFAA050069）
广西科学院基本科研业务费项目(CQZ-E-1912) （CQZ-E-1912）
广西漓江流域景观资源保育与可持续利用重点实验室研究基金项目(LRCSU21K0203) （LRCSU21K0203）
广西植物研究所基本业务费项目(桂植业23006,25007) （桂植业23006,25007）
广西喀斯特植物保育与恢复生态学重点实验室基金项目(22-035-26). （22-035-26）
