老年医学与保健2025，Vol.31Issue(2)：454-459,6.
基于单细胞测序探讨针刺足三里穴治疗哮喘作用机制的实验研究
An experimental study on mechanism of acupuncture at Zusanli acupoint in treatment of asthma based on single-cell sequencing
吕笑地 1魏颖 1王文倩1
作者信息
- 1. 复旦大学附属华山医院中西医结合科,上海 200040||复旦大学中西医结合研究院,上海 200040
- 折叠
摘要
Abstract
Objective Asthma is a significant global public health issue,and its prevention and treatment strategies re-quire urgent optimization.The coexistence of multiple diseases in elderly asthma patients significantly increases the difficulty of clinical management,and the side effects of existing drug therapies have prompted researchers to actively explore new treatment targets and intervention models.Based on the therapeutic advantages demonstrated by acupuncture therapy in clinical practice of elderly asthma patients,this study intends to systematically analyze the potential correlation mechanism between acupuncture in-tervention and cell senescence regulation through single-cell transcriptome sequencing technology.Methods After a mouse asthma model was constructed,the lung tissue samples were obtained for single-cell RNA sequencing(scRNA-seq),and the Seurat and Harmony software packages were used for data quality control and batch correction.By integrating asthma genetic association data from GW AS database and expression quantitative trait loci(eQTLs)from GTEx project,the differential ex-pression analysis and Mendelian randomization(MR)analysis were used to identify asthma-related pathogenic genes.Based on the set of cell senescence characteristic genes in the GSEA database,the dynamic regulatory characteristics of acupuncture inter-vention on the expression profiles of cell senescence-related genes were systematically analyzed.Results Acupuncture could down-regulate the proportion of T cell populations,especially a part of the functional T cell subpopulation,such as the CD4+Tem cell subpopulation,thereby exerting a role in alleviating type 2 inflammation of asthma.The trend of downregulating this cell subpopulation was consistent with the trend of downregulating genes related to cell senescence.Among them,the cell se-nescence-related gene with the most definite causal relationship to asthma pathogenesis was FIGNL1 and it played an important role in the regulation of apoptosis and cell senescence.Conclusion Acupuncture at the ST36 acupoint regulates immune re-sponse through multiple pathways and targets and alleviates type 2 inflammatory response in elderly asthma patients.The down-regulation of cell senescence and the subsequent suppression of T cell subpopulation function help reduce the progression of type 2 inflammation.This mechanism has been confirmed through scRNA-seq.Future research should further explore the mechanism of action of related genes and pathways to provide a more solid theoretical basis for the application of acupuncture in the treat-ment of elderly asthma patients.关键词
老年/哮喘/细胞衰老/单细胞转录组测序/针刺/足三里Key words
elderly/asthma/cellular senescence/single-cell RNA sequencing/acupuncture/ST36引用本文复制引用
吕笑地,魏颖,王文倩..基于单细胞测序探讨针刺足三里穴治疗哮喘作用机制的实验研究[J].老年医学与保健,2025,31(2):454-459,6.
