老年医学与保健2025，Vol.31Issue(2)：481-486,6.
基于网络药理学和分子对接探讨健脾清热方治疗慢性萎缩性胃炎伴肠上皮化生的作用机制
Mechanism of action of Jianpi Qingre formula in treatment of chronic atrophic gastritis with intestinal metaplasia based on network pharmacology and molecular docking
摘要
Abstract
Objective To explore the mechanism of action of Jianpi Qingre formula in the treatment of chronic atrophic gastritis(CAG)with intestinal metaplasia(IM)based on network pharmacology and molecular docking.Methods The ac-tive ingredients and targets of the Jianpi Qingre formula were screened based on the Traditional Chinese Medicine Systems Phar-macology Database and Analysis Platform and the Uniprot database.The GeneCards and OMIM databases were searched to ob-tain the intersection targets of the Jianpi Qingre formula and CAG with IM.Cytoscape was used to construct the"drug-active ingredient-disease target"network.The STRING platform was used to analyze the protein-protein interaction relationship,and network topology was used to analyze the core targets.GO and KEGG functional enrichment analyses were carried out on the core targets.The molecular docking between the core targets and the active ingredients was analyzed by means of Autodock software.Results A total of 247 potential active ingredients and 353 targets of the formula were identified,and 2 673 potential disease targets for CAG with IM were obtained.Network topology analysis revealed 46 key nodes.Enrichment analysis identi-fied 247 pathways.The Jianpi Qingre formula in the treatment of CAG with IM mainly acts on signaling pathways such as AGE-RAGE signaling pathway,lipid and atherosclerosis pathway,hepatitis B pathway,and fluid shear stress and atherosclero-sis pathway.The three core ingredients(quercetin,ursolic acid and β-sitosterol)undergo molecular docking with TP53,TNF,STAT3,AKT1 and IL6,and their binding energies are all relatively low.Conclusion Jianpi Qingre formula may exert cura-tive effects on CAG with IM through multiple ingredients,multiple targets,and multiple pathways.关键词
网络药理学/分子对接/健脾清热方/慢性萎缩性胃炎/肠上皮化生Key words
network pharmacology/molecular docking/Jianpi Qingre formula/chronic atrophic gastritis/intestinal metaplasia引用本文复制引用
黄春锦,王艳富,李冀..基于网络药理学和分子对接探讨健脾清热方治疗慢性萎缩性胃炎伴肠上皮化生的作用机制[J].老年医学与保健,2025,31(2):481-486,6.基金项目
上海市医院协会医院管理课题(X2024173) （X2024173）
