林业科学2025，Vol.61Issue(5)：131-145,15.DOI:10.11707/j.1001-7488.LYKX20240636
美洲黑杨亲本及其不同林龄及生长势子代叶片糖代谢的差异
Differences in Leaf Sugar Metabolism of Populus deltoides Parents and their Hybrids with Different Growth Potentials and Different Forest Ages
摘要
Abstract
[Objective]In this study,the differences in key indicators of starch and sucrose metabolism in the leaves of Populus deltoides parents and their F1 hybrids with different growth potentials were investigated at different forest ages during critical periods of annual growth,with which the role of starch and sucrose metabolism in the formation and maintenance of growth heterosis in P.deltoides was analyzed.This study aims to provide valuable references for revealing the role of starch and sucrose metabolism in the formation and maintenance of growth heterosis in trees,as well as in high-yield hybrid breeding of poplars.[Method]With the space-for-time substitution method,the high-growth potential hybrids(H1,H2,H3)and low-growth potential hybrids(L3,L4)at 1-and 3-year-old forest,along with their male(MP)and female(FP)parents of P.deltoides were selected as the research materials.The content of starch and sucrose,as well as the activities of ADP-glucose pyrophosphorylase(AGPase),sucrose phosphate synthase(SPS),β-amylase(BAM),and sucrose synthase(decomposition direction,SS-I)in the leaves at different time points during critical growth periods(July,August,September)were measured using micro-methods.The differences in various indicators were compared among hybrids and parents,and the correlation and regression,as well as path analysis were conducted to elucidate the regulatory patterns of key indicators in starch and sucrose metabolism in the formation of different growth potentials.[Result]During the critical growth periods,high-growth potential hybrids at 1-year-old and 3-year-old forest exhibited significant high-parent heterosis(HPH)in growth traits such as tree height and diameter at breast height(DBH),and showed obvious mid-parent or high-parent heterosis in key indicators of starch and sucrose metabolism.However,the sugar metabolism characteristics that promote and maintain growth heterosis in high-growth potential hybrids of P.deltoides were different with different ages.Among them,1-year-old high-growth potential hybrids showed high-parent heterosis in the activity of daytime starch and sucrose synthesis related enzymes(AGPase and SPS),as well as nighttime starch consumption,with HPH values of 1.63％-13.47％,5.41％-16.03％,0.58％-4.44％,respectively.Moreover,AGPase and SPS activities were significantly positively correlated with the net increase of tree height and ground diameter(GD),with significantly positive direct effects(P＜0.05).Except for daytime SPS activity and nighttime sucrose consumption,which showed high-parent heterosis(HPH:0.61％-14.77％,0.29％-26.05％),the 3-year-old high-growth potential hybrids showed mid-parent heterosis(MPH)in other sugar metabolism indicators,which were 0.42％-12.23％,0.25％-12.20％,0.76％-5.20％,respectively.There were significant correlations between key sugar metabolism indicators and growth traits of 3-year-old P.deltoides,which were influenced by the month of measurement.The nighttime sucrose consumption and BAM,SPS,and AGPase activity had a decisive role on net growth in July,August,and September,respectively.These indicators showed opposite trends in low-growth potential hybrids.[Conclusion]The heterosis phenomenon in growth traits of P.deltoides is stable at both 1-year-old and 3-year-old forest,and the characteristics of sugar metabolism-related indicators during critical growth periods are closely related to the formation and maintenance of growth heterosis.The sugar metabolism strategies for maintaining growth heterosis in high-growth potential hybrids of P.deltoides with different forest ages are different.For 1-year-old forest,growth heterosis is primarily enhanced by increasing the accumulation and transport of sucrose and starch during day and night.The 3-year-old high-growth potential hybrids maintain their growth heterosis mainly through the accumulation and transport of sucrose,with this process being influenced by the month of measurement.关键词
美洲黑杨/淀粉代谢/蔗糖代谢/生长性状/杂种优势Key words
Populus deltoides/starch metabolism/sucrose metabolism/growth traits/heterosis分类
农业科技引用本文复制引用
张静,余金金,黄秦军,张伟溪,丁昌俊,褚延广,苏晓华,赵军,苏雪辉,苑正赛,李政宏..美洲黑杨亲本及其不同林龄及生长势子代叶片糖代谢的差异[J].林业科学,2025,61(5):131-145,15.基金项目
"十四五"国家重点研发计划课题(2022YFD2200301)) （2022YFD2200301）
科技创新2030—重大项目课题(2023ZD0405603) （2023ZD0405603）
国家自然科学基金面上项目(31870662). （31870662）
