农业生物技术学报2025，Vol.33Issue(6)：1181-1191,11.DOI:10.3969/j.issn.1674-7968.2025.06.001
小麦叶锈病菌效应子Pt31812的基本特性分析
Analysis on the Basic Characteristics of the Effector Pt31812 of Puccinia triticina
摘要
Abstract
Effectors play an important role in the process of pathogen invasion of plants.Understanding their functions is the key to analyzing the pathogenic mechanism of pathogenicity.In order to reveal the pathogenic mechanism of Puccinia triticina(Pt),based on the analysis of the biological information of effector Pt31812,the basic characteristics of the effector Pt31812 were analyzed using the secreting system of saccharase-deficient yeast,qRT-PCR and bacterial type Ⅲ secreting system.The results showed that Pt31812 CDS was 627 bp,encoding 208 amino acid,with 7 serine,3 threonine and 2 tyrosine potential phosphorylation sites.The N-terminus has a hydrophobic structure,1～20 amino acids were signal peptides,and had a secretory function,had no transmembrane domain,and did not act on mitochondria.It could inhibit the programmed cell death(PCD)induced by the Pseudomonas syringae pathovar.tomato strain DC3000 in wheat leaves.The gene began to express in the early stage of leaf rust infection,and the expression level was the highest at 96 and 36 h in the compatible and incompatible interactions,respectively.It could inhibit the deposition of callose and accumulation of reactive oxygen species in wheat both'Thatcher'('Tc')and'TcLr1',as well as the expression of pathogenesis-related proteins gene 1(TaPR1)and TaPR2,interfering with the host defense response.The results provide theoretical basis for further study of the pathogenic mechanism of wheat leaf rust and have guiding significance for durable resistant breeding.关键词
小麦叶锈菌/效应子/致病机制/活性氧/病程相关蛋白Key words
Puccinia triticina(Pt)/Effector/Pathogenic mechanism/Active oxygen/Pathogenesis-related proteins分类
植物保护学引用本文复制引用
温晓蕾,李建嫄,李明媛,李浩,张娜,杨文香..小麦叶锈病菌效应子Pt31812的基本特性分析[J].农业生物技术学报,2025,33(6):1181-1191,11.基金项目
国家自然科学基金(31571956 （）
32172367) （）
河北省自然科学基金(C2024204135) （C2024204135）
河北省产业体系小麦创新团队(HCT2023010205) （HCT2023010205）
