农业生物技术学报2025，Vol.33Issue(6)：1192-1205,14.DOI:10.3969/j.issn.1674-7968.2025.06.002
辣椒PR-1基因家族的全基因组鉴定及其响应辣椒疫霉的表达模式分析
Genome-wide Identification of PR-1 Gene Family in Capsicum annuum and Analysis of Its Expression Pattern in Response to Phytophthora capsici
摘要
Abstract
Pathogenesis-related protein 1(PR-1),which are defense proteins in plant-pathogen interactions,play an important role in the resistance and defense of plants against diseases.The present study aimed at genome-wide identification and bioinformatics analyses of PR-1 genes in pepper(Capsicum annuum).The analyses resulted in the identification of 19 novel CaPR-1 genes,each of which produced a protein belonging to the CAP superfamily.Most of the CaPR-1 proteins contained an N-terminal signal peptide.Phylogenetic tree analysis of a total of 54 PR-1 protein sequences from pepper,tomato(Solanum lycopersicum)and Arabidopsis thaliana showed that the PR-1 family proteins were divided into 3 categories,and the pepper PR-1 was closely related to tomato PR-1.The structural analysis revealed similar 3D structures of all CaPR-1 proteins except CaPR-1.1 and CaPR-1.12.In the promoter of CaPR-1 gene,hypothetical cis-elements related to defense stress response,plant hormones,light and transcription factor were detected.The GO and KEGG functional annotations predicted their function in the defense stress response,plant hormones and plant-pathogen interactions.Microarray data analysis showed that CaPR-1 not only responded to cold,salinity,drought and heat stresse,but also affected by exogenous hormones such as salicylic acid,methyl jasmonate,ethylene and abscisic acid.CaPR-1.4 and CaPR-1.15 genes were up-regulated under salinity or drought stress,and CaPR-1.17 gene was up-regulated inducted by 4 exogenous hormones.The expression pattern of CaPR-1 transcript was altered under infection with Phytophthora capsici,with up-regulation of alkaline CaPR-1 genes such as CaPR-1.4,CaPR-1.15 and CaPR-1.17.The drastic variation in the transcript levels of CaPR-1.17 gene was further validated through qRT-PCR and it showed a significant up-regulation in infected samples(P＜0.05).The result showed that the CaPR-1 family might be important in plant stress resistance.This study provides a reference for in-depth exploration of the mechanism of pepper PR-1 family involved in P.capsic resistance.关键词
辣椒/病程相关蛋白1(PR-1)/全基因组/辣椒疫霉Key words
Capsicum annuum/Pathogenesis related protein-1(PR-1)/Genome-wide/Phytophthora capsici分类
园艺学与植物营养学引用本文复制引用
赫卫,张慧,王嘉琳,黄越,王兴亮,申晓宇..辣椒PR-1基因家族的全基因组鉴定及其响应辣椒疫霉的表达模式分析[J].农业生物技术学报,2025,33(6):1192-1205,14.基金项目
贵州中医药大学博士启动基金[(2020)43号] （2020）
黑龙江省重点研发计划项目(2022ZX02B07) （2022ZX02B07）
山东省重点研发计划(农业良种工程)项目(2024LZGCQY009) （农业良种工程）
