农业生物技术学报2025，Vol.33Issue(6)：1206-1219,14.DOI:10.3969/j.issn.1674-7968.2025.06.003
绿豆Trihelix基因家族的鉴定及表达特征分析
Identification and Expression Characterization Analysis of Trihelix Gene Family in Mung Bean(Vigna radiata)
摘要
Abstract
Trihelix transcription factor is a plant-specific transcription factor that plays an important role in plant seed formation,flower development,light regulation and stress resistance.In order to analyze Trihelix transcription factor role in the growth and development process and stress response,38 Trihelix family members(VrGT)were identified in the genome of Mung bean(Vigna radiata)via bioinformatic analyses,which were unevenly distributed on 1～10 chromosome and classified into 5 sub-families.Members of the same sub-family shared the similar motifs types and quantities,and contained specific conserved motifs.Most of the VrGT genes contained 1～2 introns.Fragment duplication was the main driver of the expansion of the VrGT genes.There were 12 and 30 VrGT genes that were interspecific collinearity with Arabidopsis thaliana and soybean(Glycine max)genomes,respectively.Numerous of cis-acting elements related to plant growth and development,light responsiveness,hormones responsiveness and stress responsiveness were predicted in the promoter regions(-2000 bp)of the VrGT genes.Multiple transcription factor families,such as WRKY,MYB and bHLH,were coexpressed with VrGT genes.The relative expression patterns of 38 VrGT genes were different at different growth stages,in different tissues of plants and under different abiotic stresses,among which the expression level was the highest in germinated seeds and 4 genes had high expression levels during the growth and development process of mung bean.There were 3 genes that had a high response level in the early and late stages of drought stress,and 8 genes that had a significant regulatory effect under salinity-alkalinity stress.This study provides a reference for further understanding the biological function of VrGT family in mung bean.关键词
绿豆/Trihelix转录因子/干旱胁迫/盐碱胁迫/表达特征分析Key words
Mung bean/Trihelix transcription factor/Drought stress/Salinity-alkalinity stress/Expression characterization analysis分类
农业科学引用本文复制引用
李梦蛟,邢展宁,余清,刘飞,邢宝龙,刘支平..绿豆Trihelix基因家族的鉴定及表达特征分析[J].农业生物技术学报,2025,33(6):1206-1219,14.基金项目
山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目(SXBYKY2022090) （SXBYKY2022090）
国家食用豆产业技术体系大同综合试验站(CARS-08) （CARS-08）
大同市应用基础研究计划(2022059) （2022059）
国家重点研发计划子课题(2021YFD1600602-07) （2021YFD1600602-07）
山西农业大学生物育种工程(YZGC077) （YZGC077）
山西省后稷实验室自主立项课题(202304010930003-12) （202304010930003-12）
山西省基础研究计划(202203021212440) （202203021212440）
