农业生物技术学报2025，Vol.33Issue(6)：1234-1245,12.DOI:10.3969/j.issn.1674-7968.2025.06.005
基于转录组测序的甜瓜DNAJ基因家族鉴定与表达分析
Identification and Expression Analysis of DNAJ Gene Family in Melon(Cucumis melo)Based on Transcriptome Sequencing
摘要
Abstract
DNAJ protein usually plays an important role in plant growth and development and resistance to biological and non-biological stress as an auxiliary companion molecule of heat shock protein70(HSP70).A total of 10 melon(Cucumis melo)DNAJ candidate members were identified in the transcriptome sequencing data of melon seedlings after low-temperature treatment by bioinformatics methods in this study.Furthermore,chromosome position,gene structure,subcellular localization,protein secondary/tertiary structure were determined.Meanwhile,the protein interaction,systematic evolution,cis-acting element and transcription level under abiotic stresses were analyzed.Results showed that 4 CmDNAJ members were distributed on 3 chromosomes,and were named CmDNAJ1～CmDNAJ4.The length of amino acids ranged from 161 to 503 aa,all of which were hydrophilic,and located in the nucleus(CmDNAJ2/3),cytoplasmic(CmDNAJ12/3/4)and endoplasmic reticulum(CmDNAJ2).Systematic evolutionary analysis found that members of the melon DNAJ gene family were closely related to cucumber(C.sativus).The predictive analysis found that the 4 DNAJ promoters mainly contained cis-acting elements such as hormone response,light response and abiotic stress response.There were significant differences in tissue-specific expression of the 4 genes,only CmDNAJ1 and CmDNAJ2 showing highly expressed in male flowers,female flowers,roots,fruits and leaves of melons.CmDNAJ1,CmDNAJ2 and CmDNAJ3 might play a positive regulatory role in the process of fruit development and maturity,while CmDNAJ4 might play a negative regulatory role.The results of qRT-PCR confirmed that CmDNAJ1～CmDNAJ4 all responded to low temperature,high salt,abscisic acid(ABA),and drought induction.CmDNAJ1 and CmDNAJ2 were the key gene for responding to cold stress;CmDNAJ2 and CmDNAJ3 were the key genes for responding to salt stress,ABA stress and drought stress.This study provides a theoretical reference for analyzing the DNAJ gene function of melon.关键词
甜瓜/热激蛋白/非生物胁迫/表达分析Key words
Melon/Heat shock protein/Abiotic stress/Expression analysis分类
园艺学与植物营养学引用本文复制引用
汪涛,嵇泽琳,杜清洁,李娟起,汪虎,王吉庆,肖怀娟,李猛..基于转录组测序的甜瓜DNAJ基因家族鉴定与表达分析[J].农业生物技术学报,2025,33(6):1234-1245,12.基金项目
国家自然科学基金(32202578) （32202578）
中国博士后科学基金(2022M711063) （2022M711063）
河南省大宗蔬菜产业技术体系岗位专家项目(HARS-22-07-G4) （HARS-22-07-G4）
