农业生物技术学报2025，Vol.33Issue(6)：1270-1279,10.DOI:10.3969/j.issn.1674-7968.2025.06.008
脱氧雪腐镰刀菌烯醇通过激活TXNIP/NLRP3炎性小体通路诱导IPEC-J2细胞炎性反应
Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating TXNIP/NLRP3 Inflammasome Pathway
摘要
Abstract
Deoxynivalenol(DON)is one of the most serious mycotoxins which severely contaminates grains and their products.Exposure to DON causes animal poisoning,and its primary target organ is the intestine.To explore the connection and mechanisms involved in the intestinal inflammatory response triggered by DON and its interaction with the NOD-like receptor signaling pathway,in this study,porcine intestinal epithelial cells(IPEC-J2)were cultured with 1 μg/mL DON.ELISA was used to quantify the concentration of interleukin-1β(IL-1β)in the cell supernatants.The expression levels of thioredoxin interaction protein(TXNIP),cysteinyl aspartate specific proteinase-1 P20(Caspase-1 P20)and NOD-like receptor protein 3(NLRP3)were detected by Western blot.TXNIP siRNA transfection was used to further verify the regulation of NLRP3 by TXNIP.Antioxidant N-acetyl-cysteine(NAC)was used to detect the interaction among reactive oxygen species(ROS)and NLRP3 inflammasome activation.The results showed that compared with blank control group,the secretion of IL-1β in the cell supernatant was extremely significantly elevated in the DON-treated group.(P＜0.01),and the protein expressions of TXNIP/NLRP3 inflammasome pathway-related proteins TXNIP,NLRP3 and Caspase-1 P20 in the cells were significantly up-regulated(P＜0.05).Compared with negative transfection in DON-treated group,the secretion of IL-1β in the supernatant of TXNIP siRNA transfection in DON-treated group was extremely significantly decreased(P＜0.01),and the protein expression of Caspase-1 P20 in the cells was significantly down-regulated(P＜0.05).Compared with DON-treated group,the NAC intervention group exhibited significantly reduced cellular ROS levels(P＜0.05),decreased secretion of IL-1β in the supernatant,and significantly downregulated expression of TXNIP and Caspase-1 P20 protein levels(P＜0.05)in the cells.This study showed that DON induced the inflammatory response of IPEC-J2 cells through the TXNIP/NLRP3 inflammasome pathway,and the antioxidant NAC could alleviate the activation of NLRP3 induced by DON.This study identifies novel therapeutic targets and offers new insights for the effective prevention and management of DON-induced toxicity.关键词
脱氧雪腐镰刀菌烯醇(DON)/炎性反应/NOD样受体蛋白3(NLRP3)炎性小体/硫氧还蛋白互作蛋白(TXNIP)Key words
Deoxynivalenol(DON)/Inflammation/NOD-like receptor protein 3(NLRP3)inflammasome/Thioredoxin interaction protein(TXNIP)分类
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周闯,夏荧,丁硕,王星迪,魏筱君,时璐,张华..脱氧雪腐镰刀菌烯醇通过激活TXNIP/NLRP3炎性小体通路诱导IPEC-J2细胞炎性反应[J].农业生物技术学报,2025,33(6):1270-1279,10.基金项目
江苏农林职业技术学院科技计划项目(2024kj33) （2024kj33）
2025年甘肃省高校研究生"创新之星"项目(2025CXZX-770) （2025CXZX-770）
省部级-江苏省高等学校基础科学(自然科学)面上项目(21KJB230002) （自然科学）
