农业生物技术学报2025，Vol.33Issue(6)：1280-1289,10.DOI:10.3969/j.issn.1674-7968.2025.06.009
IGF-1、IGF-2和TGF-β2在不同年龄牦牛肺脏中的表达分析
Expression Analysis of IGF-1,IGF-2 and TGF-β2 in the Lung of Yaks(Bos grunniens)in Different Ages
摘要
Abstract
Insulin like growth factor(IGF)IGF-1 and IGF-2,as hypoxia target factors,can cooperate with transforming growth factor-β2(TGF-β2)to participate in lung growth and development,immune regulation and injury repair.The purpose of this study was to investigate the expression and distribution characteristics of IGF-1,IGF-2 and TGF-β2 in the lungs of yaks(Bos grunniens)in different ages.The distribution and expression levels of IGF-1,IGF-2 and TGF-β2 in the lungs of yaks at different ages were investigated by immunohistochemical staining,qRT-PCR and Western blot.Immunohistochemical analysis showed that the expression positions of the three growth factors in the yak lungs were similar,and they were mainly distributed in all levels of bronchial epithelial cells and alveolar epithelial cells.In addition,IGF-1 and IGF-2 were also expressed in pulmonary artery endothelial cells.The results of qRT-PCR showed that the expression levels of IGF-1 and TGF-β2 genes in the lungs of adult yaks were significantly higher than those of newborn,juvenile and elderly yaks(P＜0.05).The expression of IGF-2 gene in the lung of newborn yak was significantly higher than that in other age groups(P＜0.05).The results of Western blot showed that the expression of IGF-1 and IGF-2 protein was the highest in the primary stage and significantly higher than that in other age groups(P＜0.05).The expression of TGF-β2 protein was the highest in adulthood,and was significantly higher than that in other age groups(P＜0.05).The expression levels of the 3 factors were similar with age,and the expression levels were higher in newborn and adulthood,suggesting that IGF-1,IGF-2,and TGF-β2 might be involved in the development of lung and the formation of hypoxic adaptive structure in yak at this age.This study provides the basis for exploring the specific adaptation mechanism of yak lung in hypoxic environment.关键词
牦牛/肺脏/胰岛素样生长因子1(IGF-1)/IGF-2/转化生长因子β2(TGF-β2)Key words
Yak/Lung/Insulin like growth factor 1(IGF-1)/IGF-2/Transforming growth factor-β2(TGF-β2)分类
畜牧业引用本文复制引用
邓演江,陈春燕,钟董兰,崔燕,余四九,魏鹏强,张虔,牛悦悦,鲜康乐,王登慧..IGF-1、IGF-2和TGF-β2在不同年龄牦牛肺脏中的表达分析[J].农业生物技术学报,2025,33(6):1280-1289,10.基金项目
国家自然科学基金(32372974) （32372974）
