人民珠江2025，Vol.46Issue(5)：85-99,15.DOI:10.3969/j.issn.1001-9235.2025.05.009
基于围隔实验探究无机电子供体对好氧反硝化菌群脱氮效能的强化
Augmentation of Nitrogen Removal Efficiency of Aerobic Denitrifying Bacterial Communities by Inorganic Electron Donors via Enclosure Experiments
摘要
Abstract
During the long-term operation of reservoirs,significant accumulation of pollutants occurs under static hydraulic conditions,profoundly influencing the distribution of nitrogen,phosphorus,organic matter,and other contaminants in water bodies.This study addressed the nitrogen pollution issue in the Jinpen Reservoir of the Heihe River by employing sponge iron-activated carbon as an inorganic electron donor and polyurethane sponge as a microbial carrier to investigate aerobic denitrification for nitrogen removal.Further analysis of microbial community structure and quantitative polymerase chain reaction(qPCR)were conducted to explore the characteristics of inorganic electron donor-driven aerobic denitrification for nitrogen removal.The results demonstrate that water pollutants are continuously reduced during the operation of the pilot enclosure system.In the first operational cycle(40 days),the total nitrogen concentration decreases from 1.39 mg/L to 0.847 mg/L,achieving a removal rate of 39.06%,while the NO3--N concentration declines from 1.077 mg/L to 0.761 mg/L,corresponding to a removal rate of 29.34%.During the second cycle,the total nitrogen concentration decreases from 2.591 mg/L to 1.895 mg/L,representing a reduction of 0.696 mg/L and a removal rate of 26.86%.Similarly,the NO3--N concentration drops from 2.559 mg/L to 1.849 mg/L,with a removal rate of 27.74%.These findings confirm the efficacy of the pilot system in eliminating water pollutants.Additionally,high-throughput sequencing and absolute quantitative analyses reveal that system operation alters microbial distribution and synergistic interactions,stimulating the expression of functional enzymes critical to pollutant degradation.This study provides a feasible strategy for in-situ bioremediation of contaminated water bodies.关键词
好氧反硝化/无机电子供体/水源水库/微生物群落结构/原位修复Key words
aerobic denitrification/inorganic electron donor/water source reservoir/microbial community structure/in-situ bioremediation分类
水利科学引用本文复制引用
贾俊杰,颜慈航,黄廷林,邢振焱,龚慧..基于围隔实验探究无机电子供体对好氧反硝化菌群脱氮效能的强化[J].人民珠江,2025,46(5):85-99,15.基金项目
陕西省重点科技创新团队项目(2023-CX-TD-32) （2023-CX-TD-32）
陕西省秦创原"科学家+工程师"队伍建设(2023KXJ-227) （2023KXJ-227）
