农业环境科学学报2025，Vol.44Issue(4)：1029-1045,17.DOI:10.11654/jaes.2024-0199
不同植被对煤矿区土壤微生物和碳循环基因的影响
Effects of different vegetation on soil microorganisms and carbon cycling genes in mining areas
摘要
Abstract
In order to clarify the microbial community characteristics under different vegetation types in Hongliulin coal mine,and to explore the influence of soil microbes and carbon cycle functional genes in the mining area by vegetation types.In this study,we selected three typical vegetation types of herbaceous(C),shrubs(G)and grass-shrub mixed(CG)soils(0-20 cm),rhizosphere soils(20-60 cm)and deep soils(60-100 cm)in the Hongliulin coal mine area of Shenmu,northern Shaanxi Province,to determine soil physicochemical properties and enzyme activities,and analyse changes in microbial community structure and carbon cycle functional gene abundance by means of high-throughput 16S rDNA,ITS rDNA sequencing and macro-genome sequencing technologies.The changes in microbial community structure and abundance of carbon cycle genes were analysed with the help of 16S rDNA,ITS rDNA high-throughput sequencing and macro-genome sequencing technologies.Results showed different vegetation types significantly affected the physicochemical properties and enzyme activities of the degraded soils in the mining area.The hydrolytic enzymes were higher in the shrub type,while the oxidoreductase enzymes were highest in the grass-shrub mixed type.Microbial α-diversity varied significantly among different vegetation types and depths,while microbial β-diversity and the relative abundance of species at the gate level varied significantly.Different vegetation types may affect the abundance of functional genes in the carbon cycle process by influencing the indicators of physical and chemical properties such as TN,AP,AK,sucrase activity,and the indicators of organic carbon fractions such as POC,ROC,and MBC.The study showed different vegetation types in the coal mine area of northern Shaanxi have significant effects on the soil microbial community structure and carbon cycle process-related functional genes,and the shrub type dominated by red willow is more suitable for improving the microbial structure and carbon cycle in the coal mine area.关键词
煤矿区/植被类型/土壤酶活性/微生物群落/碳循环/功能基因Key words
coal mining areas/vegetation type/soil enzyme activity/microbial community/carbon cycle/functional gene分类
农业科技引用本文复制引用
白轶伟,刘长月,原建强,李鹏飞,郭俏,王旭东,来航线..不同植被对煤矿区土壤微生物和碳循环基因的影响[J].农业环境科学学报,2025,44(4):1029-1045,17.基金项目
陕煤集团神木红柳林矿业公司集团级科研项目(2020SMHKJ-A-J-03-02/02)Group-level Scientific Research Project of Shenmu Hongliulin Mining Company of Shaanxi Coal Group,China(2020SMHKJ-A-J-03-02/02) （2020SMHKJ-A-J-03-02/02）
