世界科学技术-中医药现代化2025，Vol.27Issue(4)：982-991,10.DOI:10.11842/wst.20240616001
电针联合芍药甘草汤抑制卒中大鼠肢体痉挛
Shaoyao Gancao Decoction Combined with Electroacupuncture Against Poststroke Spasticity in Rats
摘要
Abstract
Objective To observe the effect of Shaoyao Gancao decoction combined with electroacupuncture on poststroke spasticity in rats.Methods Healthy male Sprague-Dawley(SD)rats were randomly divided into blank group,model group,electroacupuncture group,baclofen group,electroacupuncture combined with high dose of Chinese medicine group,electroacupuncture combined with middle dose of Chinese medicine group,electroacupuncture combined with low dose of Chinese medicine group,high dose of Chinese medicine group,middle dose of Chinese medicine group and low dose of Chinese medicine group,with 10 rats in each group.Open field test and integrated electromyography of quadriceps femoris were tested before modeling and on the 1st,3rd and 6th day after modeling.The samples were taken on day 6.The expression of inflammatory factors in M1 cortex were detected by liquid phase factor technique.The expression of brain-derived neurotrophic factor(BDNF),synaptophysin(SYP),postsynaptic density 95(PSD95),Nestin and β-catenin in M1 cortex was detected by ELISA and Western blot.Results Compared with model group and other intervention groups,the electroacupuncture combined with middle dose of Chinese medicine group make best total distance(P=0.021)and average speed(P=0.021)in open field test on 6th day.make better in integrated electromyography of quadriceps femoris test(P=0.006)on day 3,have higher Ⅱ type fiber ratio,promote IL-10(P=0.006),GMcsf(P=0.045)secretion.ELISA and Western blot also showed that this group behave better in the test of BDNF,SYP,PSD95,Nestin and β-catenin.Conclusion Electroacupuncture combined with Shaoyao-Gancao decoction groups were effective in the treatment of poststroke spasticity in rats,and its mechanism may be to improve the inflammatory environment of the injured site,promote the proliferation of neural stem cells and synaptic regeneration.关键词
电针/芍药甘草汤/阳陵泉/曲池/脑卒中/肢体痉挛Key words
Electroacupuncture/Shaoyao Gancao decoction/Yanglingquan/Qu Chi/Stroke/Limb spasticity分类
医药卫生引用本文复制引用
尚颖,黄雅琪,王菲,赵海龙,张生富,许能贵,郭斌..电针联合芍药甘草汤抑制卒中大鼠肢体痉挛[J].世界科学技术-中医药现代化,2025,27(4):982-991,10.基金项目
国家自然科学基金委员会地区科学基金项目(82260967):基于内源性干细胞激活分化探讨电针结合芍药甘草汤调节脑卒中肢体痉挛状态大鼠M1区神经元重塑的作用机制,负责人:郭斌 （82260967）
国家自然科学基金委员会地区科学基金项目(82460972):基于小胶质细胞M1/M2极化探索针药结合调节脑卒中肢体痉挛大鼠大脑内神经可塑的作用机制,负责人:郭斌 （82460972）
宁夏回族自治区自然科学基金优秀青年项目(2022AAC05030):电针结合芍药甘草汤调节脑卒中肢体痉挛状态大鼠神经可塑的机制研究,负责人:郭斌. （2022AAC05030）
