现代检验医学杂志2025，Vol.40Issue(3)：158-163,6.DOI:10.3969/j.issn.1671-7414.2025.03.029
儿童泌尿系统感染患者尿液分离大肠埃希菌的毒力基因及抗生素耐药分析
Virulence Gene and Antimicrobial Resistance Analysis of E.coli Isolated from Urine of Children with Urinary System Infection
摘要
Abstract
Objective To analyze the virulence genes and antibiotics resistance of Escherichia coli(E.coli)isolates cause urinary tract infection(USI)in children,and further understand the epidemiological characteristics of E.coli isolates in children with USI.Methods Children with urinary system infection admitted to Quanzhou Maternity and Child Health Care Hospital&Children's Hospital from January to December 2021 were collected.E.coli was isolated from urine,and the drug sensitivity of the E.coli to 15 commonly used clinical antibiotics was detected by instruments.Among them,33 strains of E.coli that showed Extended-spectrum beta-lactamase(ESBL)production were increased,genomic DNA was extracted,and the whole genome sequence of the strains was analyzed by high-throughput sequencing technology.Results In the gene sequence analysis of 33 strains of E.coli,86 virulence genes were obtained,and the bacterial structural virulence genes accounted for the most(43/86),including capsular,fimbriae and cell-membrane system,followed by functional virulence genes and toxigenic genes,while fimH,fdeC and terC virulence genes had the highest detection rates.In the analysis of drug resistance,Cefazolin(96.97%)was the most resistant,and carbapenems were also found to be resistant.In addition,39 inactivation genes,18 target alteration genes and 51 efflux system genes were obtained.The most information related to antibiotic efflux(1 273/1 815),mainly RND and MFS superfamily efflux pumps.There were 19～43 drug resistance genes in each E.coli genome.A variety of β-lactamase resistance genes were found,in which CTX-M/EC/TEM gene family dominated.Conclusion In the urine isolation of ESBL-producing E.coli in USI children,the β-lactamase should continue to be monitored.Virulence genes fimH,fdeC,terC and acrAB/emrAB/mdtABC-TolC efflux pump gene detected higherratio,should be more attention.关键词
儿童/泌尿系统感染/大肠埃希菌/毒力基因/抗生素Key words
children/urinary system infection/E.coli/virulence gene/drug-resistance分类
医药卫生引用本文复制引用
彭维林,黄慰慰,王茵娜,雷莹,杨惠鑫..儿童泌尿系统感染患者尿液分离大肠埃希菌的毒力基因及抗生素耐药分析[J].现代检验医学杂志,2025,40(3):158-163,6.基金项目
泉州市科技计划项目(No.2020C016R). （No.2020C016R）
