生物技术通报2025，Vol.41Issue(5)：82-89,8.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1246
控制水稻光响应基因ELM1的图位克隆
Map-based Cloning of Light-responsive Gene ELM1 in Rice
摘要
Abstract
[Objective]Exploring the regulatory genes of rice flowering date and conducting positional cloning research,the genetic and molecular mechanisms of rice flowering can be clarified,the regulatory network of rice heading date genes can be improved,and certain theoretical and practical significance can be provided for rice production and breeding.[Method]Using the late-flowering mutant elm1 under long-day conditions in rice as the material,we analyzed agronomic traits such as flowering time.Then we constructed a population through reciprocal crosses and analyzed the genetic patterns by phenotyping the F2 population.Further we also developed a mapping population with the indica rice Dular for positional cloning and gene localization.Finally,we sequenced the candidate genes within the fine-mapped interval,predicted and analyzed the candidate genes using bioinformatics tools,and predicted the changes in protein structure using AlphaFold2 software.[Result]The elm1 mutant demonstrated a significantly prolonged flowering time under long-day conditions,which was controlled by a single recessive nuclear gene.The ELM1 gene through positional cloning was cloned.The ELM1 gene encoded a SET-domain histone methyltransferase and was allelic to the previously reported lvp1 mutant.Sequencing results showed that a point mutation(G to T)in the elm1 mutant occurred in the 5th exon of the LOC_Os09g13740 gene(3 293 bp downstream of the ATG start codon),resulting in a change from glycine(hydrophilic)to valine(hydrophobic)at this site.Protein structure prediction using AlphaFold2 indicated that this mutation caused a conformational change in the protein,which may affect its function.Field experiments showed that it showed a phenotype with significantly increased number of grains per panicle and higher yield when the elm1 mutant was grown in suitable latitudinal regions.[Conclusion]The mutation site in the elm1 mutant is a weak allele.Breeding this allelic mutant in suitable latitudinal regions may enhance rice yield.Further studies have demonstrated that the elm1 mutant is an excellent allelic variation material.关键词
水稻/开花期/elm1突变体/图位克隆/弱突变/蛋白结构Key words
rice/heading date/elm1 mutant/map-based cloning/weak mutation/protein structure引用本文复制引用
杜量衡,唐黄磊,张治国..控制水稻光响应基因ELM1的图位克隆[J].生物技术通报,2025,41(5):82-89,8.基金项目
国家重点研发计划项目(2022YFF1001700) （2022YFF1001700）
