生物技术通报2025，Vol.41Issue(5)：90-103,14.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0979
高粱TGA基因响应孢堆黑粉菌(Sporisorium reilianum)侵染表达和DNA变异分析
Analysis on Expression and DNA Variation of TGA Genes in Sorghum(Sorghum bicolor)in Response to Sporisorium reilianum Infection
摘要
Abstract
[Objective]The TGA family is a very important group of bZIP transcription factors.This study is aimed to identify the TGA gene family members of the sorghum genome,analyse gene expression and natural allele variation analysis,and screen the genes and cis-acting elements that respond to the infection of Sporisorium reilianum.[Method]We identified the members of SbTGAs gene family in the whole genome through bioinformatic and molecular biological methods,and analysed their chromosome location,gene structure,protein physicochemical properties,evolutionary relationship,cis-regulatory elements of their putative promoters,collinearity analysis,and transcriptional expression characteristics after the infection of head smut fungus.[Result]In sorghum genome there were 17 SbTGA family members,all of them contained the DOG1 domain and most carried bZIP domain.All SbTGAs were located in the nucleus,and most of the SbTGA gene had 3‒12 introns.Phylogenetic analysis showed that the TGA gene family could be divided into five subfamilies.There were 15 genes in Zea mays having collinearity with sorghum,and their genetic relationships were closer.Gene expression analysis showed that the expressions of SbTGA2.1b were significant in the roots and leaves,and significantly induced after infection by S.reilianum in both lines with resistance and susceptability to head smut.It can be seen that the expressions of SbTGA2.2,SbTGA5 and SbTGA6.6g in healthy lines were up-regulated compared with that in susceptible lines.The synthetic analysis of whole genome resequencing data revealed five genes,including SbTGA2.1a and SbTGA6.6g,carrying multiple non-synonymous SNP and INDEL variations.[Conclusion]SbTGAs including SbTGA2.1b are hypothesized to associate with sorghum head smut resistance.These findings provide an evidences for breeding sorghum for resistance to head smut.关键词
高粱/TGA/孢堆黑粉菌/基因表达/DNA变异Key words
sorghum/TGA/head smut fungus Sporisorium reilianum/gene expression/DNA variation引用本文复制引用
刘卓君,赵威军,吕晋慧,张春来,柴文婷,任毅乐,王新宇,朱立勋,赵珊珊,杨博慧,范佳利,李新凤..高粱TGA基因响应孢堆黑粉菌(Sporisorium reilianum)侵染表达和DNA变异分析[J].生物技术通报,2025,41(5):90-103,14.基金项目
国家自然科学基金项目(31971994,31470285),山西省教育厅研究生实践创新(2023SJ109),山西农科院博士基金项目(ybsjj1404),山西省教育厅重点专业建设项目(J20220211) （31971994,31470285）
