生物技术通报2025，Vol.41Issue(5)：119-128,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1171
马铃薯StAS2-15基因的克隆及盐胁迫下功能分析
Cloning and Functional Analysis of the StAS2-15 Gene in Potato under Salt Stress
摘要
Abstract
[Objective]The ASYMMETRIC LEAVES2(AS2)gene family is a plant-specific transcription family that plays an important role in plant growth and development and response to stress.The study of the function and mechanism of AS2 transcription factors in potato in response to adversity stress,especially salt stress,may provide theoretical and technical support for breeding new salt-resistant potato varieties.[Method]We cloned the StAS2-15 gene from the leaves of wild-type potato variety DES,constructed the StAS2-15 overexpression vector by homologous recombination,and transformed DES to obtain positive plants for salt-resistant agronomic traits and physiological and biochemical experiments.[Result]Salt stress phenotyping experiments showed that plant height,fresh weight,root number and root length of overexpressed plants(OEs)and wild type(DES)significantly decreased with increasing salt concentration,in which plant height decreased by 2.42%-43.21%and 28.77%-58.49%,respectively;and plant height,fresh weight,root number and root length of OEs significantly increased compared with those of DES at the same salt concentration.Superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)activities and proline content in the leaves of positive plants overexpressing StAS2-15 gene were significantly higher and malondialdehyde(MDA)content was significantly lower than that of the wild type under different salt concentration treatments.The qRT-PCR results showed that the expressions of potato stress-related response genes ABI3,MYB2,and SnRK2s in overexpressed plants was significantly higher than that in wild type under salt stress.The RT-qPCR results showed that the expressions of potato stress-related response genes ABI3,MYB2,and SnRK2s in overexpressed plants was significantly higher than that in wild type under salt stress.[Conclusion]The overexpression of StAS2-15 gene alteres the expressions of some antioxidant enzymes,content of osmoregulatory substances and other salt stress responsive genes in potato,and the growth status of plants under salt stress shows a desensitized phenotype,which affects the growth and development of potato.关键词
马铃薯/StAS2-15/愈伤组织转化体系/盐胁迫/RT-qPCRKey words
potato/StAS2-15/callus transformation system/salt stress/RT-qPCR引用本文复制引用
宋慧洋,苏宝杰,李京昊,梅超,宋倩娜,崔福柱,冯瑞云..马铃薯StAS2-15基因的克隆及盐胁迫下功能分析[J].生物技术通报,2025,41(5):119-128,10.基金项目
山西省基础研究计划项目(202203021211272),山西省重点研发计划项目(202102140601004),山西农业大学农学院育种工程专项(YZ2021-04),马铃薯遗传改良与种质创新山西省重点实验室项目(202304010921003),山西省现代农业产业技术体系建设专项资金(2024CYJSTX06-10) （202203021211272）
