生物技术通报2025，Vol.41Issue(5)：141-152,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0745
基于T2T基因组鉴定大豆R2R3-MYB基因家族及干旱和盐胁迫下的表达分析
Identification of the Soybean R2R3-MYB Gene Family Based on the T2T Genome and Their Expression Analysis under Drought and Salt Stress
摘要
Abstract
[Objective]R2R3-MYB transcription factors play an important role in plant response to stress conditions.It is aimed to fully identify the R2R3-MYB transcription factors in soybean and understand the role of soybean R2R3-MYB genes in stress responses.[Method]The soybean R2R3-MYB family genes were systematically identified and evolutionarily analyzed using the soybean Telomere-to-Telomere(T2T)genome.Real-time fluorescence quantitative PCR(RT-qPCR)was used to analyze the expression of this family of genes under drought and salt stress conditions.[Result]The soybean genome contains 324 R2R3-MYB genes,77 of which were newly identified and distributed on 20 chromosomes.The results of evolutionary analysis showed that soybean R2R3-MYB family genes can be divided into four subgroups,and the colinear gene pairs were closer on the branches of the evolutionary tree.Promoter analysis revealed that most cis-acting elements related to ABA and drought resistance were present.Through expression analysis,10 genes were speculated to be involved in the process of soybean salt tolerance,of which GmMYB19 and GmMYB76 have been reported,and GmMYB89 and GmMYB214 were identified for the first time and found to be related to soybean drought and salt tolerance.[Conclusion]A total of 321 R2R3-MYB family genes were identified in soybean,and 10 candidate genes for drought and salt tolerance were identified through analysis of their expression patterns under drought and salt stress conditions.关键词
大豆/R2R3-MYB基因家族/生物信息学分析/干旱和盐胁迫/表达分析/T2TKey words
Glycine max/R2R3-MYB gene family/bioinformatics analysis/drought and salt stress/expression analysis/T2T引用本文复制引用
李志强,罗正乾,徐琳黎,周国慧,屈丝雨,刘恩良,顼东婷..基于T2T基因组鉴定大豆R2R3-MYB基因家族及干旱和盐胁迫下的表达分析[J].生物技术通报,2025,41(5):141-152,12.基金项目
新疆维吾尔自治区自然科学基金资助项目(2023D01B17) （2023D01B17）
