生物技术通报2025，Vol.41Issue(5)：165-174,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1066
2,4-表油菜素内酯对镉胁迫下胡萝卜幼苗生理特性的影响
Effects of Exogenous 2,4-Epibrassinolide on Physiological Characteristics of Daucus carota L.Seedlings under Cadmium Stress
摘要
Abstract
[Objective]This study is aimed to investigate the impact of varying concentrations of 2,4-epibrassinolide on growth indices,physiological parameters,and the expression of cadmium(Cd)uptake and transporter-related genes in carrot(Daucus carota L.)seedlings subjected to Cd stress.The findings provide theoretical insights into Cd resistance research in carrots.[Method]The"Hongxin 4"carrot cultivar(Daucus carota L.)was selected for the experimental materials.Using a hydroponic system,we examined the effects of different concentrations(0.1,0.5,1,and 1.5 μmol/L)of 2,4-epibrassinolide on the growth traits,physiological indicators,and photosynthesis of carrot seedlings under 0.1 mmol/L CdCl2 stress.[Result]The growth of carrot seedlings was significantly inhibited under 0.1 mmol/L Cd stress.However,the exogenous application of 1 μmol/L 2,4-epibrassinolide inhibited cadmium ion absorption and transport,promoted the root length and plant height,elevated the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and ascorbate peroxidase(APX)in the leaves,increased the contents of photosynthetic pigments,enhanced the photosynthetic efficiency,and suppressed the expressions of Cd uptake and transport-related genes(DcHMA3,DcHMA4,DcCAX2 and DcCAX4),thereby maintaining the balance of redox metabolic pathways in the plants.[Conclusion]2,4-epibrassinolide promoted carrot root growth,enhanced antioxidant enzyme activity,improved photosynthetic efficiency,regulated the glutathione-phytochelatin(GSH-PC)metabolic pathway,and modulated the expressions of related genes to alleviate Cd stress-induced damage in carrot seedlings.The optimal concentration of 2,4-epibrassinolid for carrots was found to be 1 μmol/L.关键词
镉胁迫/2,4-表油菜素内酯/胡萝卜/生长和生理特性/基因表达Key words
cadmium stress/2,4-epibrassinolide/carrot/physiological characteristics/gene expression引用本文复制引用
周志国,樊双虎,邓晨,冯雪..2,4-表油菜素内酯对镉胁迫下胡萝卜幼苗生理特性的影响[J].生物技术通报,2025,41(5):165-174,10.基金项目
河北省教育厅青年拔尖人才项目(BJK2024187) （BJK2024187）
