生物技术通报2025，Vol.41Issue(5)：186-196,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1174
兰州百合三个LdBBXs基因的克隆与表达分析
Cloning and Expression Analysis of Three LdBBXs in Lilium davidii var.willmottiae
摘要
Abstract
[Objective]Members of the BBX subgroup Ⅳ play an important role in regulating anthocyanins synthesis in response to light.The BBX gene of lily was cloned and its response to different light times and its effect on the reddening of scales were analyzed,which may provide important candidate genes for breeding Lilium davidii var.willmottiae varieties with scales keeping white upon light exposure.[Method]Three members of BBX subgroup Ⅳ(LdBBX21,LdBBX22,and LdBBX24)were cloned from L.davidii var.willmottia according to its transcriptome data,and the bioinformatics analysis and subcellular localization analysis of the proteins encoded by them were performed,and their expression patterns in different tissues and scales after light treatment were studied by quantitative real-time polymerase chain reaction(RT-qPCR).[Result]LdBBX21,LdBBX22,and LdBBX24 all contained two B-box conserved domains,and belonged to BBX subgroup Ⅳ.The results of subcellular localization showed that LdBBX21,LdBBX22 and LdBBX24 were all localized in the nucleus.Tissue-specific expression analysis showed that LdBBX22 and LdBBX24 had the highest expression in flowers and the lowest in scales,while LdBBX21 showed the opposite expression pattern.RT-qPCR analysis showed that in scales treated with different light duration,the gene expression of LdBBX21 showed a downward trend with the increase of light duration;the gene expression of LdBBX22 increased with the increase of light,which was basically the same as the change trend of anthocyanin content;the expression of LdBBX24 increased and then decreased of light duration.[Conclusion]All of LdBBX21,LdBBX22,and LdBBX24 may play important functions in light-regulated anthocyanin glycoside synthesis in lily scales,laying the foundation for subsequent functional verification of the LdBBX gene.关键词
兰州百合/BBX/基因克隆/表达分析Key words
Lilium davidii var.willmottiae/BBX/gene cloning/expression analysis引用本文复制引用
刘鑫,王嘉雯,李进伟,牟策,杨盼盼,明军,徐雷锋..兰州百合三个LdBBXs基因的克隆与表达分析[J].生物技术通报,2025,41(5):186-196,11.基金项目
国家自然科学基金项目(32172624) （32172624）
