生物技术通报2025，Vol.41Issue(5)：244-254,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1086
蒺藜苜蓿MtZHD4基因克隆、亚细胞定位及表达分析
Cloning,Subcellular Localization and Expression Analysis of MtZHD4 Gene from Medicago truncatula
摘要
Abstract
[Objective]This study aims to clone and analyze the expression characteristics of the Medicago truncatula MtZHD4 gene,in order to gain a deeper understanding of its function,and to provide a theoretical basis for its involvement in the growth and development of M.truncatula,as well as in the synthesis,regulation,and signaling of plant endogenous hormones.[Method]The M.truncatula cultivar'R108'was used as the material.The MtZHD4 gene was cloned,and bioinformatics analysis and subcellular localization were performed.Real-time quantitative PCR(qPCR)was employed to analyze the expression pattern of MtZHD4 gene in different tissues,hormones,and stress treatments.[Result]The open reading frame(ORF)of the MtZHD4 gene was 1 092 bp,encoding a protein of 363 amino acids.Prediction of the conserved domains revealed that MtZHD4 contained two ZF-HD protein domains,which belonged to the ZF-HD dimmer and homeo ZF-HD superfamilies.Subcellular localization indicated that MtZHD4 was located in the nucleus and cytoplasm of the cell.Analysis of promoter cis-acting element showed that the promoter of MtZHD4 contained multiple cis-elements involved in plant growth and development,hormone response,and light response.The results of tissue-specific expression analysis revealed that the expressions of MtZHD4 varied significantly across different tissues,with the highest expression in the roots and lower levels in the leaves and pods.After treatment with different hormones and stress conditions,the expression of the MtZHD4 gene showed an upregulation trend under ABA induction,a downregulation trend under IAA induction,and a pattern of first increasing and then decreasing under 6-BA,MeJA,and SA induction.Under salt stress,the expression of the MtZHD4 gene increased,while under drought stress,the expression first increased and then decreased.[Conclusion]MtZHD4 gene responds to different hormones and abiotic stresses,and may regulate the growth and development of M.truncatula through related hormone signaling pathways,and play a positive regulatory role under salt and drought stress.关键词
蒺藜苜蓿/ZF-HD转录因子/生物信息学分析/亚细胞定位/组织差异/激素处理/盐胁迫/干旱胁迫Key words
Medicago truncatula/ZF-HD transcription factor/bioinformatics analysis/subcellular localization/tissue differences/hormone treatment/salt stress/drought stress引用本文复制引用
杨春,王晓倩,王红军,晁跃辉..蒺藜苜蓿MtZHD4基因克隆、亚细胞定位及表达分析[J].生物技术通报,2025,41(5):244-254,11.基金项目
内蒙古自治区科技厅内蒙古自治区科技重大专项项目(2022JBGS00160302) （2022JBGS00160302）
