水土保持通报2025，Vol.45Issue(2)：64-72,9.DOI:10.13961/j.cnki.stbctb.2025.02.008
灌溉方式对设施黄瓜氮素气态损失及氮循环功能基因的影响
Nitrogen gaseous loss and functional genes of nitrogen cycling in greenhouse cucumbers under different irrigation methods
摘要
Abstract
[Objectives]The effects of different irrigation treatments on gaseous nitrogen loss and nitrogen cycle functional genes in a greenhouse cucumber ecosystem were explored to provide data support for further studies on gaseous nitrogen loss in a greenhouse farmland system under different irrigation methods.The results provide a theoretical basis for the formulation of irrigation schemes in greenhouse vegetable production systems in China.[Methods]Facility cucumbers were chosen as the research subjects for field experiments.The experimental designs included traditional irrigation(100％IWD),25％water-saving irrigation(75％IWD),and 50％water-saving irrigation(50％IWD).Through in situ field monitoring experiments,continuous observations and records of N2O emissions and ammonia volatilization from the soil of facility cucumbers were made using static chamber-gas chromatography and ventilation absorption.Nitrogen cycle functional genes were identified using high-throughput qPCR gene chip technology.[Results]① Among the various irrigation treatments,the cumulative emissions of N2O were as follows:100％IWD＞75％IWD(p＜0.05)and 100％IWD＞50％IWD(p＜0.05).② Cumulative ammonia volatilization was 75％IWD＞50％IWD＞100％IWD(p＜0.05).③ The overall abundance of nitrogen cycle functional genes in the soil changed significantly(PERMANOVA,F=241.82,p=0.001).④ Cucumber yield was 100％IWD＞75％IWD＞50％IWD(p＜0.05).[Conclusion]Irrigation at 50％significantly increased nitrification gene abundance,whereas 75％and 50％irrigation significantly decreased denitrification gene abundance.Denitrification was the main factor affecting N2O gas production in surface soil treated with 100％irrigation.Reducing the irrigation water volume by 25％led to a decrease in denitrification gene abundance in the surface soil and weakened the denitrification process in the surface soil,reducing N2O emissions.However,reducing irrigation water by 50％did not significantly change denitrification gene abundance or N2O emissions at the soil surface.关键词
设施黄瓜/灌溉/氧化亚氮/氨挥发/氮循环功能基因Key words
greenhouse cucumber/irrigation/nitrous oxide/ammonia volatilization/functional genes of nitrogen cycling分类
农业科技引用本文复制引用
万博,吴林亿,周其文,杜君,魏义长,王丽丽..灌溉方式对设施黄瓜氮素气态损失及氮循环功能基因的影响[J].水土保持通报,2025,45(2):64-72,9.基金项目
国家重点研发计划"黄淮海北部集约化农区氮素面源污染发生过程与调控机制"(2021YFD1700903) （2021YFD1700903）
