西南农业学报2025，Vol.38Issue(3)：429-440,12.DOI:10.16213/j.cnki.scjas.2025.3.001
烤烟间作大豆对烤烟根际土壤微生物多样性的影响
Effect of roasted tobacco intercropping with soybean on microbial diversity of tobacco inter-root soil
摘要
Abstract
[Objective]The study aims to analyze the changes of inter-root soil physicochemical properties and microbial community structure and composition of roasted tobacco under intercropping soybean mode,which will provide the scientific basis for future optimization of the in-tercropping mode and intercropping cultivation management of roasted tobacco.[Method]The materials that included tobacco intercropping soils of CK,ID1 and ID2 were used to analyze the physical and chemical properties of tobacco intercropping soils and the structure of micro-bial communities in tobacco intercropping soils.[Result]Soybean intercropping significantly increased organic matter(OM)content,alka-line dissolved nitrogen(AN),rapid phosphorus(AP)and fast-acting potassium(AK)in tobacco inter-root soils.According to the analysis of Alpha diversity,the Ace index,Chao index,Shannon index and Shannon's equal diversity index of tobacco inter-root soil fungi were high-er than those of CK in ID1 and ID2 treatments,the Ace index and Chao index in particular(P＜0.05).The analysis of species Venn dia-gram revealed that compared with CK treatment,the total soil bacterial AS Vs in the inter-root soil of tobacco increased by 13.26％ and 2.81％,and the endemic bacterial ASVs raised by 7.85％ and 25.30％,respectively.In addition,the inter-root soil fungal ASVs saw the increment of 29.63％ and 25.04％,and the endemic fungal ASVs witnessed the increment of 59.22％ and 49.02％.The proportion of dominant fungi such as Actinobacteria and Ascomycota in ID1 and ID2 treatments was higher than that in control(CK),and Kickxellomyco-ta,the endemic phylum,appeared in the inter-root soil fungal community of roasted tobacco treated with intercropped soybean.PICRUSt 2 function prediction analysis showed that compared with roasted tobacco monoculture,intercropped soybean enhanced the function of carbohy-drate and amino acid metabolism related pathways in inter-root bacteria compared with tobacco monoculture.Functional prediction by FUN-Guild fungi showed that intercropping soybean reduced the number of phytopathogenic bacteria in the soil.[Conclusion]Intercropping soy-bean plays a significant role in increasing the content of OM,AN,AP and AK in the inter-root soil of roasted tobacco,enhancing the Ace and Chao indices of inter-root microorganisms and improving the richness and diversity of microorganisms in the inter-root soil of roasted to-bacco.关键词
烟草/大豆/间作/土壤微生物Key words
Tobacco/Soybean/Intercropping/Soil microorganisms分类
农业科技引用本文复制引用
牛燕蕙,胡颖梅,高明博,杜兴华,段菲,李浩,Thiri HAN,海江波,白银萍..烤烟间作大豆对烤烟根际土壤微生物多样性的影响[J].西南农业学报,2025,38(3):429-440,12.基金项目
中国烟草总公司陕西省公司科技项目(K4050724019) （K4050724019）
