西南农业学报2025，Vol.38Issue(3)：519-528,10.DOI:10.16213/j.cnki.scjas.2025.3.009
干旱胁迫下小麦抗旱性评价及代谢组学分析
Evaluation of drought resistance and metabolomic analysis of wheat under drought stress
摘要
Abstract
[Objective]The present paper aimed to investigate the drought resistance mechanism of wheat variety Longyu 10 at both physiolog-ical and metabolomic levels,and at the same time to provide theoretical basis for the popularization of the cultivation of this variety.[Method]Bainong4199 was used as a control variety,and drought treatment was carried out at seedling stage and adult stage to determine the growth and physiological indexes of the two wheat varieties,and the metabolites and metabolic pathways were determined by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)technology.[Result]The germination rate,germination energy,germina-tion index,coleoptile length,seedling height and chlorophyll content of the two wheat varieties were significantly reduced during the seedling stage at drought treatment,with a smaller decrease in Longyu 10,however,the content of malondialdehyde,proline,soluble sugar,soluble pro-tein,superoxide dismutase activity,and peroxidase activity of the two wheat varieties were significantly increased,with a larger increase in Longyu 10.The changes of physiological indexes at drought stress in the adult plant stage were similar to those in the seedling stage,and the plant height and flag leaf area of Longyu 10 were reduced under drought stress,but the reduction of Bainong 4199 reached a significant level.The spikelet number,grain number spike,and 1000-grain weight of the two varieties were all reduced under drought stress,among which the reduction of the spikelet number reached a significant level,but the grain number spike was not significant,and the 1000-grain weight of Bai-nong 4199 reached a significant level.In addition,the expression levels of metabolites such as amino acids and their derivatives and organic acids changed significantly after drought stress by UPLC-MS/MS detection,and the metabolites of caffeine metabolism pathway and zeatin bi-osynthesis pathway were specifically up-regulated in Longyu 10.[Conclusion]Longyu 10 has a stronger drought resistance,and the upregula-tion of amino acid metabolites,alkaloids,and nucleotide sugar content may be the basis for its response to drought stress.关键词
小麦/干旱胁迫/生理指标/代谢组学Key words
Wheat/Drought stress/Physiological index/Metabolomics分类
农业科技引用本文复制引用
段珊,祁伟亮,魏子尧,张流珍,陈会兰,王秋雨,施万喜..干旱胁迫下小麦抗旱性评价及代谢组学分析[J].西南农业学报,2025,38(3):519-528,10.基金项目
陇东学院博士基金项目(XYBYZK2205、XYBYZK2107) （XYBYZK2205、XYBYZK2107）
甘肃省教育厅产业支撑计划项目(2023CYZC-67) （2023CYZC-67）
国家自然科学基金项目(32360455) （32360455）
