天津农业科学2025，Vol.31Issue(3)：27-35,9.DOI:10.3969/j.issn.1006-6500.2025.03.005
牛骨骼肌卫星细胞发育过程中的转录组学分析
Transcriptomic Analysis during the Development of Bovine Skeletal Muscle Satellite Cells
摘要
Abstract
In order to explore the molecular mechanism of proliferation and differentiation of bovine skeletal muscle satellite cells and search for skeletal muscle associated genes in different periods,this study conducted transcriptomic sequencing analysis of bovine skeletal muscle satellite cells at proliferation and differentiation stages,and used bioinformation technology to analyze and screen functional genes.The results showed that a total of 645 differentially expressed genes were obtained,including 388 up-regulated genes,most of which were related to muscle fiber development.There were 257 down-regulated genes,mostly genes related to DNA synthesis.The difference level of differentially expressed genes,GO analysis,KEGG analysis and PPI protein interaction analysis showed that MYL1,MYH3,TNNC2,TNNT3 and TNNI2 were important candidate genes affecting muscle development in bovine skeletal muscle satellite cells.CDC6,KIF2C,PLK1,KIF11,MCM3,MCM4,MCM5 and MCM7 were important candidate genes af-fecting DNA replication in bovine skeletal muscle satellite cells.In this study,5 differentially expressed genes were screened out,which were involved in biological processes such as skeletal muscle contraction.8 differentially expressed genes were screened during proliferation,which were involved in biological processes such as DNA unwinding and DNA replication initiation.The above genes may play an important role in the proliferation and differentiation of skeletal muscle satellite cells and lay a theoretical foundation for beef cattle breeding.关键词
牛/转录组学/差异基因/骨骼肌卫星细胞Key words
bovine/transcriptomics/differential genes/skeletal muscle satellite cells分类
畜牧业引用本文复制引用
许译文,万琼飞,石闪闪,张鑫扬,董飞飏,李新..牛骨骼肌卫星细胞发育过程中的转录组学分析[J].天津农业科学,2025,31(3):27-35,9.基金项目
国家自然科学基金(31501939) （31501939）
天津市大学生创新创业训练计划项目(202310061214) （202310061214）
天津市中央引导地方科技发展资金优秀农业科技特派员项目(23ZYCGSN00400) （23ZYCGSN00400）
