天津师范大学学报（自然科学版）2025，Vol.45Issue(2)：32-40,9.DOI:10.19638/j.issn1671-1114.20250206
圈养生境下灰鹤、灰冕鹤和蓑羽鹤肠道菌群的比较
Comparison on gut microbiota among Grus grus,Balearica regulorum and Grus virgo living in captivity
摘要
Abstract
To explore the structural composition characteristics of gut microbiota and its relationship with the health level of the host,the fecal samples of three crane species(Grus grus,Balearica regulorum and Grus virgo)in captivity were collected by noninvasive sampling method.16S rRNA high-throughput sequencing technology was applied to make comparison on interspe-cific differences on gut microbiota.The results demonstrated that:① The common dominant phyla of the three species included Firmicutes,Actinobacteriota and Proteobacteria,the common dominant genus was Lactobacillus,and significant interspecific differences were found at both phylum and genus levels;② The diversity and richness of the intestinal flora of Grus grus sample groups were higher than those of the other two crane species sample groups;③ Based on the KEGG database,the functional prediction results of the intestinal flora showed that the relative abundance of global and overview,energy metabolism,amino metabolism,and cofactor and vitamin metabolism in the Grus grus sample group were higher than those in the Balearica regulo-rum and Grus virgo sample groups;④ The preliminary prediction of potential pathogenic bacteria found that the top three phyla of pathogenic bacteria in relative abundance were Proteobacteria,Firmicutes,and Patescibacteria,and the top three genera of pathogenic bacteria in relative abundance were Staphylococcus,Mesorhizobium,and Archangium.关键词
鹤科/肠道菌群/种间差异/高通量测序技术Key words
Gruidae/gut microbiota/interspecific differences/high-throughput sequencing分类
生物学引用本文复制引用
孙梦璘,吴楠,张泽明,姚红玉,吴弘,赵大鹏..圈养生境下灰鹤、灰冕鹤和蓑羽鹤肠道菌群的比较[J].天津师范大学学报（自然科学版）,2025,45(2):32-40,9.基金项目
中央财政林业补助资金项目-野生动物监测专项 （）
生态环境部生物多样性调查、观测与评估项目 （）
国家自然科学基金资助项目(31772468). （31772468）
