天津师范大学学报（自然科学版）2025，Vol.45Issue(2)：49-53,5.DOI:10.19638/j.issn1671-1114.20250208
BA-ELISA法快速检测农产品中阿特拉津残留
Rapid determination of atrazine residues in agricultural products by BA-ELISA
摘要
Abstract
To quickly determine atrazine residues in agricultural products,based on the monoclonal antibody,an indirect competitive biotin/avidin-enzyme-linked immunosorbent assay(BA-ELISA)was constructed.Cucumber,corn and sugarcane were selected as samples,and the three samples were extracted with acetonitrile.The extracted liquid was purified by PSA,steamed to nearly dry,and the volume was fixed with methanol.The extract was diluted in different multiples to eliminate the influence of complex matrix.The content of atrazine in samples was determined by BA-ELISA method and compared with GC method.The results indicated that in the optimal reaction system,the detection limit of BA-ELISA was 0.52±0.13 μg/L,and the sensitivity was 12.24±1.28 μg/L.In BA-ELISA,the recovery rate of atrazine in the three samples ranged from 93.07％to 99.67％,the coefficient of variation ranged from 1.5％to 6.7％,and the detection time was about 1 h.The results of this method are basically consistent with those of GC method,so the rapid determination of atrazine residues in agricultural prod-ucts can be achieved by BA-ELISA.关键词
阿特拉津/快速测定/生物素/亲和素酶联免疫(BA-ELISA)Key words
atrazine/rapid determination/biotin/avidin-enzyme-linked immunosorbent assay(BA-ELISA)分类
化学引用本文复制引用
胡刘岭,金靓怡,李瑞韩,刘重阳,宋洋..BA-ELISA法快速检测农产品中阿特拉津残留[J].天津师范大学学报（自然科学版）,2025,45(2):49-53,5.基金项目
天津市自然科学基金资助项目(18JCQNJC84400) （18JCQNJC84400）
国家自然科学基金青年科学基金资助项目(31301487) （31301487）
天津师范大学研究生科研创新项目(2024KYCX111Y) （2024KYCX111Y）
天津市科技计划项目(13JCQNJC14800). （13JCQNJC14800）
