云南化工2025，Vol.52Issue(5)：88-91,4.DOI:10.3969/j.issn.1004-275X.2025.05.19
HPLC法测定昭通金银花中酚酸类成分含量
Determination of Phenolic Acid Components in Lonicerae Japonicae Flos from Zhaotong by HPLC Method
摘要
Abstract
[Objective]To Develop an HPLC method to determine the content of phenolic acids in five different varieties ofLonicerae Japonicae Flos from the Zhaotong region,in order to assess the quality of various honeysuckle samples.[Methods]High-performance liquid chromatography with a C18 column was used to establish a method for determining the content of five phenolic acids,after validating the methodology,the samples were tested.[Results]The content of phenolic acids in all five varieties was higher than 3.2％,with the No.4"King of Flowers"sample,exhib-iting the highest phenolic acid content,totaling 5.9％;among all theLonicerae Japonicae Flos varieties,the content of chlorogenic acid and iso-chlorogenic acid A was the highest.[Conclusions]The method effectively separated five phenolic acids in Lonicerae Japonicae Flos,and the No.4"King of Flowers"sample had a relatively high content of phenolic acids,providing a basis for the promotion and cultivation of honeysuckle in the Zhaotong region.关键词
金银花/HPLC/酚酸类成分/绿原酸/异绿原酸AKey words
Lonicerae Japonicae Flos/HPLC/Phenolic Acid Components/Chlorogenic Acid/Isochlorogenic Acid A分类
化学引用本文复制引用
杨桂英,成孟华,秦绍焕,刘元剑..HPLC法测定昭通金银花中酚酸类成分含量[J].云南化工,2025,52(5):88-91,4.基金项目
昭通卫生职业学院2024年度校级课题(202429),课题名称:昭通不同品种金银花多成分含量研究. （202429）
