摘要
Abstract
Objective To analysa the correlation between the mRNA expression of potassium voltage-gated channel subfamily F member 1(KCNF1)in lung cancer tissue and clinical pathological features and prognosis.Methods 102 patients with lung cancer in Shexian People's Hospital and the First Affiliated Hospital of Bengbu Medical University from December 2019 to December 2021 were selected as the research object.All patients were divided into metastasis group(n=34)and non metastasis group(n=68)according to whether the patients had lung cancer metastasis.Compared the expression of KCNF1 protein and mRNA in lung cancer tissues and adjacent normal tissues.The influencing factors of metastasis of lung cancer were analyzed by univariate analysis.Spearman correlation was used to analyze the relationship between KCNF1mRNA expression and metastasis related factors.Analyzed the independent correlation between the expression of KCNF1 mRNA and the metastasis of lung cancer.Used survival curve analysis to investigate the relationship between KCNF1 protein levels and 3-year survival of lung cancer.Results The high expression rate of KCNF1 protein in lung cancer tissues was 38.24%(39/102),which was significantly higher than 7.84%(8/102)in adjacent normal tissues(χ2=26.568,P<0.001).The relative expression levels of KCNF1 mRNA in lung cancer tissues were significantly higher than those in adjacent normal tissues,the difference was statistically significant(t=23.127,P<0.001).Compared with the non metastatic group,the proportion of high and moderate differentiation in the metastatic group was higher,the proportion of TNM stages III to IV,KCNF1,Epidermal Growth Factor Receptor(EGFR),C-erbB-2,the mRNA expression levels of chemokine receptor 2(CXCR2),matrix metalloproteinase-9(MMP-9),vascular endothelial growth factor(VEGF)and HIF-l α were significantly increased,the differences were statistically significant(χ2/t=3.036～19.555,all P<0.05).The expression of KCNF1mRNA in lung cancer tissues was independently correlated with the metastasis of lung cancer.The expression of KCNF1mRNA and the transfer related factor EGFR,C-erbB-2,CXCR2,MMP-9,the expression of VEGF and HIF-l α was positively correlated(r=0.633～0.803,all P<0.05).The 3-year survival rate of patients with high expression of KCNF1 protein was significantly shorter than that of patients with low expression(HR=1.85,95%CI:1.04～3.27,P=0.032).Conclusion KCNF1mRNA and protein are highly expressed in lung cancer tissues,and its expression level is closely related to the metastasis and prognosis of patients with lung cancer,which is a risk factor for predicting the metastasis of lung cancer.关键词
电压门控钾通道亚家族F成员1/肺癌/转移Key words
potassium voltage-gated channel subfamily F member 1/lung cancer/metastasis分类
临床医学
