摘要
Abstract
Objective To investigate the correlation between polymorphisms at the rs6420094 locus of the soute carrier family 34 member1(SLC34A1)gene and renal injury in patients with H-type hypertension.Methods 128 patients with H-type hypertension admitted to Daqing Longnan Hospital of Heilongjiang Province from December 2022 to December 2023 were selected as the study group,and the patients with H-type hypertension were further categorized into the injury group(n=50)and the non-injury group(n=78)according to whether or not the renal injury occurred.Another 100 cases of normal healthy medical checkups in the same period were selected as the control group.Collected clinical data of patients,the genotype of rs6420094 locus of the SLC34A1 gene was detected by polymerase chain reaction(PCR),and the distribution frequency of genotype and allele locus were compared among the groups.Binary Logistic regression analysis of factors influencing renal injury in H-type hypertension patients.Real-time fluorescence quantitative PCR(qRT PCR)was used to detect the expression level of SLC34A1.The Pearson method analyzed the correlation between SLC34A1 expression level and clinical indicators in H-type hypertensive kidney injury patients.Results Compared with the non-injured group,Hcy(15.57±3.62 μmol/L vs 13.31±2.34 μmol/L),SCr(116.03±19.94 μmol/L vs 98.52±17.65 μmol/L),urinary β2-MG(0.86±0.25 mg/L vs 0.59±0.21 mg/L),Cys-C(2.23±0.67 mg/L vs 1.03±0.28 mg/L)were elevated,and eGFR(86.63±9.34 ml/min/1.73m2 vs 101.36±12.65 ml/min/1.73m2)was decreased,with statistically significant differences(t=4.293～14.043,all P<0.05).The genotype distributions in the control group and study group conforms to Hardy-Weinberg genetic equilibrium(χ2=0.082,0.076,all P>0.05).The frequencies of alleles and genotypes of the rs6420094 locus of the SLC34A1 gene in the control,injury and non-injury groups were significantly different(χ2=6.421,13.050,all P<0.05).Allele G as well as dominant model AG+GG were significantly lower in the injury group compared to the control group(χ2=5.699,10.125,all P<0.05).Compared with the injury group,allele G and dominant model AG+GG were significantly higher in the non-injury group(χ2=5.079,8.412,all P<0.05).Binary Logistic regression analysis showed that carrying allele G or dominant model(AA vs AG+GG)was a protective factor against the risk of renal injury in patients with type H hypertension(P<0.05).QRT-PCR results showed that SLC34A1 mRNA expression levels were significantly higher in the control group(1.68±0.33)than in the study group(1.38±0.21),and significantly higher in the non-injury group than in the injury group(1.43±0.29 vs 1.29±0.21),and the differences were statistically significant(t=8.349,2.952,all P<0.05).Pearson correlation analysis shows that,the relationship between SLC34A1 expression levels were negatively correlated with Hcy,SCr,urinary β2-MG,Cys-C(r=-0.694～-0.621,all P<0.05),and positively correlated with eGFR(r=0.692,P<0.05).Conclusion Carrying the allele G or dominant model(AG+GG)of the SLC34A1 gene rs6420094 locus in patients with H-type hypertension reduces the risk of renal injury.The expression level of SLC34A1 in patients with H-type hypertensive renal injury is decreased.关键词
H型高血压/溶质载体家庭34成员1/基因多态性/肾损伤Key words
H-type hypertension/solute carrier family 34 member 1/genetic polymorphism/kidney injury分类
医药卫生
