空军军医大学学报2025，Vol.46Issue(5)：591-597,604,8.DOI:10.13276/j.issn.2097-1656.2025.05.005
关中平原蜱虫立克次体与嗜吞噬细胞无形体本底研究
Background study on Rickettsia and Anaplasma phagocytophilum in ticks in the Guanzhong Plain
摘要
Abstract
Objective To investigate the species and geographical distribution characteristics of ticks in the Guanzhong Plain,and to determine the endemic characteristics and genetic diversity of ticks carrying Rickettsia and Anaplasma phagocytophilum(AP).Methods Tick samples were collected from May 2023 to July 2024 and DNA was extracted.Nested PCR was used to screen for Rickettsia and AP for further phylogenetic analysis.Results A total of 10 130 ticks were captured,and 219 ticks were selected for pathogen-carrying testing.Tick species identification confirmed that the detected ticks were Haemaphysalis longicornis.Pathogen detection results showed that the prevalence of Rickettsia and AP was 8.68％and 35.62％,respectively.Phylogenetic analysis showed that there were numerous variants of Rickettsia and AP in the Guanzhong Plain.The sequence of one Rickettsia strain did not cluster with the known reference sequence,suggesting that the strain may belong to a new variant strain.Conclusion The Guanzhong Plain has a high carrying rate of Rickettsia and AP,and these two pathogens can be divided into several different clades.This discovery reveals the potential diversity of their pathogenicity and provides a basis for further investigation of the pathogenicity of different clades.关键词
长角血蜱/一代测序/立克次体/嗜吞噬细胞无形体/关中平原/分子流行病学Key words
Haemaphysalis longicornis/first-generation sequencing/Rickettsia/Anaplasma phagocytophilum/Guanzhong Plain/molecular epidemiology分类
医药卫生引用本文复制引用
王玉华,刘嘉成,徐琳莉,贺真,陆振华,邵中军,刘昆,龙泳..关中平原蜱虫立克次体与嗜吞噬细胞无形体本底研究[J].空军军医大学学报,2025,46(5):591-597,604,8.基金项目
国家自然科学基金(82273689) （82273689）
