西北农业学报2025,Vol.34Issue(5):779-785,7.DOI:10.7606/j.issn.1004-1389.2025.05.001
珍稀鱼类多鳞白甲鱼的全基因组单核苷酸多态性发现及遗传多样性评价
Genome-wide SNP Discovery and Genetic Diversity Assessment of Rare Fish Species Onychostoma macrolepis
摘要
Abstract
The aim of this studyis to evaluate the genetic diversity of Onychostoma macrolepis,a spe-cies of national secondary protected wildlife.In this study,restriction site-associated DNA sequencing(RAD-seq)was used to identify 425 205 genome-wide high-confidence population single nucleotide polymorphisms(SNPs)across two populations on the northern and southern slopes of the Qinling Mountains.The expected heterozygosity,nucleotide diversity and polymorphism information content of O.macrolepis on the north slope of the Qinling Mountains were 0.177,0.200 and 0.145,respec-tively,while these three parameters of O.macrolepis on the south slope of the Qinling Mountains were 0.261,0.291 and 0.212,respectively.The population structure analysis revealed a clear contrast in genetic diversity between the two geographical populations.关键词
遗传多样性/多鳞白甲鱼/群体遗传学/限制性内切位点相关DNA测序/单核苷酸多态性Key words
Genetic diversity/Onychostoma macrolepis/Population genetics/RAD-seq/SNP引用本文复制引用
苟妮娜,靳铁治,王开锋,杨斌..珍稀鱼类多鳞白甲鱼的全基因组单核苷酸多态性发现及遗传多样性评价[J].西北农业学报,2025,34(5):779-785,7.基金项目
陕西省重点研发计划(2022NY-105). Key Research and Development Program of Shaanxi Province(No.2022NY-105). (2022NY-105)