摘要
Abstract
Objective To investigate the relationship between the expression level of disulfide bond A oxidoreductase-like protein(DsbA-L)in peripheral blood CD4+T cells of patients with acute pancreatitis(AP)and the severity of disease and prognosis of patients.Methods 172 cases of AP patients attending the Fushun County People's Hospital from January 2022 to December 2023 were selected as the study group.According to the degree of severity of the disease,AP patients were divided into mild AP group(n=64),moderately severe AP group(n=48)and severe AP group(n=60).60 patients with chronic pancreatitis(CP)were selected as the CP group.100 cases of healthy people who had physical examinations in the same period were selected as the control group.Quantitative real-time PCR(qRT-PCR)was used to detect the expression level of DsbA-L mRNA in peripheral blood CD4+T in each group;Pearson's method was used to analyze the correlation between the level of DsbA-L and the severity of the disease of the patients with AP.Multifactorial Logistic regression analysis of factors influencing the prognosis of AP patients.Results CRP,BUN,Scr,PCT,APACHE II score and BISAP score increased sequentially,and the difference was statistically significant(F=3.298～323.587,all P<0.05).The study group(0.82±0.16)DsbA-L mRNA expression level was lower than that in the control group(1.08±0.21)and CP group(1.05±0.20),and the differences were statistically significant(t=11.254,8.350,all P<0.001).DsbA-L mRNA in the moderately severe AP group(0.83±0.17)and severe AP group(0.73±0.15)expression levels were lower than those in the mild AP group(0.92±0.20),the expression level of DsbA-L mRNA in the severe AP group was significantly lower than that in the moderate to severe AP group and the differences were statistically significant(t=2.686,6.024,3.244,all P<0.05).Pearson correlation analysis,DsbA-L levels were negatively correlated with CRP,BUN,PCT,APACHE II score and BISAP score(r=-0.693～-0.591,all P<0.001).DsbA-L mRNA expression level was lower in the poor prognosis group compared with the good prognosis group(0.98±0.23 vs 0.76±0.19),and the difference was statistically significant(t=6.399,P<0.001).Multivariate Logistic regression analysis,the reduced level of DsbA-L was an independent risk factor for poor prognosis in AP patients(Waldχ2=6.757,P<0.05).Conclusion Reduced levels of DsbA-L expression within CD4+T in peripheral blood of AP patients are closely correlated with disease severity and prognosis,and can be used as a marker to assess disease severity and prognosis of AP patients.关键词
急性胰腺炎/二硫键A氧化还原酶样蛋白/病情严重程度/CD4+T细胞Key words
acute pancreatitis/disulfide bond A oxidoreductase-like protein/disease severity/CD4+T cell分类
医药卫生
